Immobilized chymotrypsin on reversibly precipitable polymerized liposome


A polymerized liposome (PLS) was prepared using a synthesized phosphatidylethanolamine with a diacetylene moiety that showed a reversibly precipitable property on addition and removal of salt. To prepare a soluble-insoluble immobilized enzyme, chymotrypsin was covalently immobilized on the outer surface of the PLS. The carbodiimide method was employed for the enzyme immobilization. Coupling was rapid and nearly complete at a weight ratio of enzyme to the PLS of < 0.12. The immobilized enzyme showed favorable activity yields for both low-and high-mol-wt substrates, i.e., 90 ±9% forN-benzoyl-L-tyrosine ethyl ester and 59 ±5% for casein up to an enzyme coupling density of 0.38 g/g-PLS. The immobilized enzyme was reusable and more stable at high temperature and long-term incubation than the native enzyme.

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Correspondence to Yan Sun.

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Sun, Y., Jin, X.H., Dong, X.Y. et al. Immobilized chymotrypsin on reversibly precipitable polymerized liposome. Appl Biochem Biotechnol 56, 331–339 (1996).

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Index Entries

  • Chymotrypsin
  • immobilization
  • polymerized liposome
  • precipitation
  • stability