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Applied Biochemistry and Biotechnology

, Volume 56, Issue 3, pp 331–339 | Cite as

Immobilized chymotrypsin on reversibly precipitable polymerized liposome

  • Yan Sun
  • X. -H. Jin
  • X. -Y. Dong
  • K. Yu
  • X. Z. Zhou
Original Articles

Abstract

A polymerized liposome (PLS) was prepared using a synthesized phosphatidylethanolamine with a diacetylene moiety that showed a reversibly precipitable property on addition and removal of salt. To prepare a soluble-insoluble immobilized enzyme, chymotrypsin was covalently immobilized on the outer surface of the PLS. The carbodiimide method was employed for the enzyme immobilization. Coupling was rapid and nearly complete at a weight ratio of enzyme to the PLS of < 0.12. The immobilized enzyme showed favorable activity yields for both low-and high-mol-wt substrates, i.e., 90 ±9% forN-benzoyl-L-tyrosine ethyl ester and 59 ±5% for casein up to an enzyme coupling density of 0.38 g/g-PLS. The immobilized enzyme was reusable and more stable at high temperature and long-term incubation than the native enzyme.

Index Entries

Chymotrypsin immobilization polymerized liposome precipitation stability 

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Copyright information

© Humana Press Inc 1996

Authors and Affiliations

  • Yan Sun
    • 1
  • X. -H. Jin
    • 1
  • X. -Y. Dong
    • 1
  • K. Yu
    • 1
  • X. Z. Zhou
    • 2
  1. 1.Department of Chemical Engineering and Research Center for BiotechnologyTianjin UniversityTianjinP. R.China
  2. 2.Department of ChemistryNankai UniversityTianjinP. R.China

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