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Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean)

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Abstract

A quantitatively main molecular form ofCratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lectin form was identified in the latter step. Iso 1 is specific for glucose/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pI range of 5.15–7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.

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References

  1. Goldstein, I. J., Hughes, R. C., Monsigny, M., Osawa, T., and Sharon, N. (1980),Nature 285, 66.

    Article  Google Scholar 

  2. Barondes, S. H. (1988),Trends Biochem. Sci. 13, 480.

    Article  CAS  Google Scholar 

  3. Sharon, N. and Lis, H. (1990),FASEB J. 4, 3198.

    CAS  Google Scholar 

  4. Nakajima, T., Yasawa, S., Kogure, T., and Furukawa, K. (1988),Biochim. Biophys. Acta 964, 207.

    CAS  Google Scholar 

  5. Ravindranaths, M., Paulson, J., and Irie, R. (1988),Cancer Res. 48, 645.

    Google Scholar 

  6. Sather, R., Leung, Y. K., Alliet, P., Lebenthal, E., and Lee, P. C. (1990),Biochim. Biophys. Acta 1051, 78.

    Article  Google Scholar 

  7. Ryder, S. D., Smith, J. A., and Rhodes, J. M. (1992),Biochim. Biophys. Acta 84, 1410.

    CAS  Google Scholar 

  8. Rinderle, S. J., Goldstein, I. J., Matta, K., and Ratcliffe, R. M. (1989),J. Biol. Chem. 264, 16123.

    CAS  Google Scholar 

  9. Cavalcanti, M. S. and Coelho, L. C. B. B. (1990),Mem. Osw. Cruz 85, 371.

    CAS  Google Scholar 

  10. Suvachittanont, W. and Pentapaiboon, A. (1992),Phytochemistry 31, 4065.

    Article  CAS  Google Scholar 

  11. Lis, H. and Sharon, N. (1973),Ann. Rev. Biochem. 42, 54.

    Google Scholar 

  12. Sharon, N. and Lis, H. (1990),FASEB J. 4, 3198.

    CAS  Google Scholar 

  13. Rodriguez, D., Cavada, B. S., Oliveira, J. T. A., Moreira, R. A., and Russo, M. (1992),Brazilian J. Med. Biol. Res. 25, 823.

    CAS  Google Scholar 

  14. Perez, G., Perez, C., Cavada, B. S., Moreira, R. A., and Richardson, M. (1991),Biochemistry 30, 2619.

    Article  CAS  Google Scholar 

  15. Moreira, R. A. and Cavada, B. S. (1984),Biol. Plant. 25, 336.

    Article  Google Scholar 

  16. Paiva, P. M. G. and Coelho, L. C. B. B. (1992),Appl. Biochem. Biotechnol. 36, 113.

    Article  CAS  Google Scholar 

  17. Sheldon, P. S. and Bowles, D. J. (1992),EMBO J. 11, 1297.

    CAS  Google Scholar 

  18. Lee, K. B. and Linhardt, R. J. (1992),Anal. Biochem. 203, 206.

    Article  CAS  Google Scholar 

  19. Mandal, D. K. and Brewer, C. F. (1992),Biochemistry 31, 12602.

    Article  CAS  Google Scholar 

  20. Bukantz, C. S. C., Rein, L. C. C. R., and Kent, J. F. (1946),J. Lab. Clin. Med. 31, 349.

    Google Scholar 

  21. Bing, D. H., Weyand, J. G. M., and Stavitsky, A. B. (1967),Proc. Soc. Cyp. Biol. Med. 124, 1166.

    CAS  Google Scholar 

  22. Shiomi, K., Kamiya, H., and Shimizu, Y. (1979),Biochim. Biophys. Acta 576, 118.

    CAS  Google Scholar 

  23. Association of Official Analytical Chemists (1990),Official Methods of Analysis of the Association of Official Analytical Chemists. 15 ed. Washington, v. 1.

  24. Reisfield, R. A., Lewis, U. J., and Williams, D. E. (1962),Nature 195, 281.

    Article  Google Scholar 

  25. Analytical Electrofocusing in Thin Layers of Polyacrylamide Gels (1977),Bromma,LKB, (LKB-application note, 250).

  26. Laemmli, U. K. (1970),Nature 227, 680.

    Article  CAS  Google Scholar 

  27. Pharmacia Fine Chemicals (1980),Polyacrylamide Gel Electrophoresis: Laboratory Techniques. Uppsala.

  28. Parish, R. W., Schmidlin, S., and Muller, U. (1977),Exp. Cell. Res. 110, 267.

    Article  CAS  Google Scholar 

  29. O’Farrell, P. H. (1975),J. Biol. Chem. 250, 4007.

    CAS  Google Scholar 

  30. Hudson, L. and Hay, F. C. (1980),Practical Immunology, 2nd ed., Blackwell, Edinburgh.

    Google Scholar 

  31. Ashford, D., Allen, A. K., and Neuberger, A. (1982),Biochem. J. 201, 641.

    CAS  Google Scholar 

  32. Weir, D. M. (1973),Handbook of Experimental Immunology, 2nd ed., Blackwell, pp. 34.24–34.25.

  33. Kolberg, J. and Sletten, K. (1982),Biochim. Biophys. Acta 704, 26.

    CAS  Google Scholar 

  34. Agrawal, B. L. and Goldstein, I. J. (1972),Methods Enzymol. Complex Carbohydrates 28, 313.

    Article  Google Scholar 

  35. Oxender, D. L. and Fox, C.F. (1987),Protein Engineering (Liss, A. R., Inc.), NY, p. 187.

    Google Scholar 

  36. Etzler, M. E.(1972),Methods Enzymol. Complex Carbohydrates 28, 340.

    Article  Google Scholar 

  37. Mock, A. and Renwrantz, L. (1991),Comp. Biochem. Physiol. 3, 699.

    Google Scholar 

  38. Kortt, A. A. (1984),J. Biochem. 138, 519.

    CAS  Google Scholar 

  39. Cammue, B. P., Peeters, B., and Peumans, W. (1985),Biochem. J. 227, 949.

    CAS  Google Scholar 

  40. Lerivray, H., Chesnel, A., and Jego, P. (1985),Comp. Biochem. Physiol. 81, 385.

    Article  Google Scholar 

  41. Kawagishi, H., and Mori, H. (1991),Biochim. Biophys. Acta 1076, 179.

    CAS  Google Scholar 

  42. Dunn, M. Y., and Burghes, A. H. M. (1983),Electrophoresis 4, 97.

    Article  CAS  Google Scholar 

  43. Gomes, Y. M., Furtado, A. F., and Coelho, L. C. B. B. (1991),Appl. Biochem. Biotechnol. 31, 97.

    Article  CAS  Google Scholar 

  44. Lis, H. and Sharon, N. (1981), inThe Biochemistry of Plants: a Comprehensive Treatise, (Marcus, A., ed.), Academic, NY, vol. 6, p. 371.

    Google Scholar 

  45. Sage, H. J. and Green, R. W. (1972),Methods Enzymol. 28, 332.

    Article  Google Scholar 

  46. Bhattacharryya, L., Das, P. K., and Sen, A. (1981),Arch. Biochem. Biophys. 211, 459.

    Article  Google Scholar 

  47. Leathern, A. (1986), inImmunocytochemistry Modern Methods and Applications, 2nd ed. (Polak, J. M. and Vannorden, S., eds.), Wright Bristol, London.

    Google Scholar 

  48. Stadtman, E.R. (1968),Ann. NY Acad. Sci. 151, 516.

    Article  CAS  Google Scholar 

  49. Morell, A. G., Gregoriadis, G., and Scheinberg, I. H. (1971),J. Biol. Chem. 246, 1461.

    CAS  Google Scholar 

  50. Moss, D. W. (1982), inIsoenzymes (Chapman and Hall Ltd.), Wright Bristol, London.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, UFPE, 50670-420, PE, Brazil

    Maria T. S. Correia & Luana C. B. B. Coelho

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  1. Maria T. S. Correia
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  2. Luana C. B. B. Coelho
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Correia, M.T.S., Coelho, L.C.B.B. Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart. (Camaratu Bean). Appl Biochem Biotechnol 55, 261–273 (1995). https://doi.org/10.1007/BF02786865

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  • DOI: https://doi.org/10.1007/BF02786865

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