Abstract
Continuous β-transelimination of citrus pectin was achieved using pectin lyase [PNL, poly(methoxygalacturonide)lyase, EC 4.2.2.10] purified fromPenicillium italicum and confined in a membrane reactor. Various operational parameters, such as enzyme and substrate concentrations, filtrate flowrate, and reaction volume were optimized. Kinetic studies indicated that β-transelimination of pectin in the continuous reactor occurred following a first-order reaction with respect to substrate concentration for up to 90 min at 1 mg/mL pectin. This time period could be extended up to 120 min when the reactor was fed with 3–4 mg/mL pectin. During the first 50 h of operation, the system was capable of maintaining a viscosity reduction of 55% below the initial value when it was continuously fed with 4 mg/mL pectin.
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Alkorta, I., Garbisu, C., Llama, M.J. et al. β-Transelimination of citrus pectin catalyzed by penicillium italicum pectin lyase in a membrane reactor. Appl Biochem Biotechnol 55, 249–259 (1995). https://doi.org/10.1007/BF02786864
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DOI: https://doi.org/10.1007/BF02786864