Effect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of panax notoginseng
- 123 Downloads
Effects of initial phosphate concentration on the growth, ginsenoside saponin production, and the consumption of sugar and nitrogen sources by suspended cells ofPanax notoginseng (Burk) F. H. Chen were investigated in a 250-mL shake flask. The results indicate that by increasing the initial phosphate concentration in the medium in the range of 0–1.25 mM, both the cell growth and the saponin accumulation were greatly improved, and the utilization of sugar and nitrogen sources was also increased. The highest production, productivity, and yield of ginsenosides obtained were 0.98 g/L, 45.5 mg/L/d, and 0.030 g/g at 1.25 mM of initial medium phosphate. At a relatively higher level of medium phosphate, i.e., 2.0 mM, the product accumulation was inhibited to some degree, although the cell growth was not.
Index EntriesCell growth ginsenoside saponin initial phosphate concentration nutrient utilization Panax notoginseng plant cell culture secondary metabolite production
Unable to display preview. Download preview PDF.
- 1.Wei, J. X. and Du, Y. Z. (1992),Natl. Prod. Res. Dev. 4, 94–100 (Chin.).Google Scholar
- 2.Ando, T., Tanaka, O., and Shibata, S. (1971),Shoyakagaku Zasshi 25(1), 28–32 (Jpn.).Google Scholar
- 3.Yunnan Bot. Inst. (1975),Acta Phytotaxon. Sin. 13, 29–44 (Chin.).Google Scholar
- 4.Zhang, G. D., Zhou, Z. H., Wang, M. Z., and Gao, F. Y. (1980),Acta Pharm. Sin. 15, 175–181 (Chin.).Google Scholar
- 5.Loo, S. W., Huang, W. H., Cao, G. Y., and Yu, R. C. (1964),Plant Physiol. Commun. 1964(2), 26, 28 (Chin.).Google Scholar
- 8.Zheng, G. Z. and Wang, S. L. (1989),Acta Bot. Yunnan 11, 255–262 (Chin.).Google Scholar
- 9.Zhou, L. G., Zheng, G. Z., Wang, S. L., Gan, F. Y., and Xu, C. (1992),Acta Bot. Sin. 34, 76–80 (Chin.).Google Scholar
- 11.Liang, S.-Z., Zhong, J.-J., and Yoshida, T. (1991),Industrial Microbiol. 21 (3), 27–31 (Chin.).Google Scholar
- 14.Tanaka, H., Takayama, S., Mano, Y., Hayashi, T., and Inoguchi, M. (1992),Shokubutsu Saibou Kogaku (Plant Cell Technology), Omu-sha, Tokyo (Jpn.).Google Scholar