Abstract
Effects of initial phosphate concentration on the growth, ginsenoside saponin production, and the consumption of sugar and nitrogen sources by suspended cells ofPanax notoginseng (Burk) F. H. Chen were investigated in a 250-mL shake flask. The results indicate that by increasing the initial phosphate concentration in the medium in the range of 0–1.25 mM, both the cell growth and the saponin accumulation were greatly improved, and the utilization of sugar and nitrogen sources was also increased. The highest production, productivity, and yield of ginsenosides obtained were 0.98 g/L, 45.5 mg/L/d, and 0.030 g/g at 1.25 mM of initial medium phosphate. At a relatively higher level of medium phosphate, i.e., 2.0 mM, the product accumulation was inhibited to some degree, although the cell growth was not.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Wei, J. X. and Du, Y. Z. (1992),Natl. Prod. Res. Dev. 4, 94–100 (Chin.).
Ando, T., Tanaka, O., and Shibata, S. (1971),Shoyakagaku Zasshi 25(1), 28–32 (Jpn.).
Yunnan Bot. Inst. (1975),Acta Phytotaxon. Sin. 13, 29–44 (Chin.).
Zhang, G. D., Zhou, Z. H., Wang, M. Z., and Gao, F. Y. (1980),Acta Pharm. Sin. 15, 175–181 (Chin.).
Loo, S. W., Huang, W. H., Cao, G. Y., and Yu, R. C. (1964),Plant Physiol. Commun. 1964(2), 26, 28 (Chin.).
Furuya, T., Yoshikawa, T., Orihara, Y., and Oda, H. (1984),J. Natl. Prod. 47, 70–75.
Asada, I., Ii, I., Hirotani, M., Asada, Y., and Furuya, T. (1993),Biotechnol. Lett. 15, 1259–1264.
Zheng, G. Z. and Wang, S. L. (1989),Acta Bot. Yunnan 11, 255–262 (Chin.).
Zhou, L. G., Zheng, G. Z., Wang, S. L., Gan, F. Y., and Xu, C. (1992),Acta Bot. Sin. 34, 76–80 (Chin.).
Dunlop, D. S. and Curtis, W. R. (1991),Biotechnol. Prog. 7, 434–438.
Liang, S.-Z., Zhong, J.-J., and Yoshida, T. (1991),Industrial Microbiol. 21 (3), 27–31 (Chin.).
Panda, A. K., Mishra, S., and Bisaria, V. S. (1992),Biotechnol. Bioeng. 39, 1043–1051.
Payne, J., Hamill, J. D., Robins, R. J., and Rhodes, M. J. C. (1987),Planta Med. 53, 474–478.
Tanaka, H., Takayama, S., Mano, Y., Hayashi, T., and Inoguchi, M. (1992),Shokubutsu Saibou Kogaku (Plant Cell Technology), Omu-sha, Tokyo (Jpn.).
Zhong, J.-J., Seki, T., Kinoshita, S., and Yoshida, T. (1991),Biotechnol. Bioeng. 38, 653–658.
Weatherburn, M. W. (1967),Anal. Chem. 39, 971–974.
Chen, P. S., Toribara, T. Y., and Warner, H. (1956),Anal. Chem. 28, 1756–1758.
Martinez, B. C. and Park, C.-H. (1993),Biotechnol. Prog. 9, 97–100.
Zhong, J.-J., Fujiyama, K., Seki, T., and Yoshida, T. (1994),Biotechnol. Bioeng. 44, 649–654.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhong, JJ., Zhu, QX. Effect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of panax notoginseng. Appl Biochem Biotechnol 55, 241–247 (1995). https://doi.org/10.1007/BF02786863
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02786863