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The phospholipase-induced degradation of phosphatidylnucleosides

Russian Journal of Bioorganic Chemistry volume 26, pages 213–215 (2000)Cite this article

Abstract

Hydrolysis of phosphatidylnucleosides, 5′-(rac-1-hexadecyl-2-palmitoyl-sn-glycero-3-phosphoryl)-3′-azido-3′-deoxythymidine and -2′,3′-didehydro-3′-deoxythymidine, effected by phospholipases (PL) A2, C, and D was studied to reveal the metabolism of these derivatives. It was shown that PLA2 deacetylates the glycerol residue at position 2, PLC is inactive, and PLD hydrolyzes the phosphatidylnucleosides to give free nucleosides.

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Abbreviations

AZT:

3′-azido-3′-deoxythymidine

ddC:

2′,3′-dideoxycytidine

ddI:

2′,3′-dideoxyinosine

d4T:

2′,3′-didehydro-3′-deoxythymidine

PLA2, PLC, and PLD:

phospholipases A2, C, and D, respectively

EPC:

egg yolk phosphatidylcholine

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Authors and Affiliations

  1. State Research Institute of Organic Chemistry and Technology, shosse Entuziastov 23, 111024, Moscow, Russia

    T. I. Novozhilova, S. I. Malekin, V. I. Kozhukhov, Yu. L. Kruglyak & V. K. Kurochkin

Authors
  1. T. I. Novozhilova
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  2. S. I. Malekin
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  3. V. I. Kozhukhov
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  4. Yu. L. Kruglyak
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  5. V. K. Kurochkin
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Novozhilova, T.I., Malekin, S.I., Kozhukhov, V.I. et al. The phospholipase-induced degradation of phosphatidylnucleosides. Russ J Bioorg Chem 26, 213–215 (2000). https://doi.org/10.1007/BF02786348

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  • DOI: https://doi.org/10.1007/BF02786348

Key words

  • anti-HIV activity
  • nucleoside
  • phosphatidylnucleoside
  • phospholipase