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A noninstrumental immunoassay based on colloidal dyes


Detecting labels based on water dispersions of colloidal textile dyes were developed that are useful in various analytical and diagnostic test systems for a simple visual assessment of the assay. Colored water-insoluble particles of dyes were used for the sorptional immobilization of streptavidin on their surface. The resulting streptavidin-dye (STR-DYE) complexes possessed a high visualizing capacity and were used for the combined detection of pesticides (simazine and 2,4-dichlorophenoxyacetic acid) by noninstrumental immunoassay (DYE-comb-assay, competitive dot-immunoassay in the comb format). The detection limits and the duration of our DYE-comb-assay (4 ng/ml, 20–25 min), HRP-comb-assay (competitive dot-immunoassay in the comb format using the enzymic conjugate of STR with horseradish peroxidase) (16 ng/ml), and the traditional competitive ELISA (12–16 ng/ml, 1.5 h) were compared. This DYE-comb-assay is simple enough and can be used under field conditions.

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borate buffer, pH 7.4


BB containing ovalbumin (2 mg/ml)


biotinylated immunoglobulins


2,4-dichlorophenoxyacetic acid


a polyvalent conjugate of 2,4-D with ovalbumin


N-2-hydroxyethylpiperazine-N-(2-ethanesulfonic acid)


horseradish peroxidase


3-(N-morpholino)propanesulfonic acid




phosphate buffered saline, pH 7.4


PBS containing ovalbumin (2 mg/ml)


PBS containing 0.05% Tween-20


PBST containing ovalbumin (2 mg/ml)




a polyvalent conjugate of simazine with ovalbumin




a complex of colloidal dye with streptavidin


a conjugate of streptavidin with horseradish peroxidase


  1. Gerbitsidy (Herbicides), Zakharenko, V.A., Ed., Moscow: Agropromizdat, 1990.

    Google Scholar 

  2. Hock, B.,Acta Hydrochim. Hydrobiol., 1993, vol. 21, pp. 71–83.

    Article  CAS  Google Scholar 

  3. Knopp, D.,Anal. Chim. Acta, 1995, vol. 311, pp. 383–392.

    Article  CAS  Google Scholar 

  4. Marco, M.-P., Gee, S., and Hammock, B.D.,Trends Anal. Chem., 1995, vol. 14, pp. 341–350.

    CAS  Google Scholar 

  5. Pavlova, I.S., Lyubavina, I.A., Zherdev, A.V., and Zinchenko, A.A.,Bioorg. Khim., 1997, vol. 23, pp. 832–838.

    PubMed  CAS  Google Scholar 

  6. Complementary Immunoassay, Collins, W.P., Ed., New York: Wiley, 1988.

    Google Scholar 

  7. Hsu, Y.,Anal. Biochem., 1984, vol. 142, pp. 221–225.

    PubMed  Article  CAS  Google Scholar 

  8. Tijssen, P. and Kurstak, E.,Anal. Biochem., 1984, vol. 136, pp. 451–457.

    PubMed  Article  CAS  Google Scholar 

  9. Kobayashi, R. and Tashima, Y.,Anal. Biochem., 1989, vol. 183, pp. 9–13.

    PubMed  Article  CAS  Google Scholar 

  10. Goodrow, M.H., Harrison, R.O., and Hammock, B.D.,J. Agric. Food Chem., 1990, vol. 38, pp. 990–996.

    Article  CAS  Google Scholar 

  11. Eremin, S.A., Lunskaya, I.M., and Egorov, A.M.,Bioorg. Khim., 1993, vol. 19, pp. 836–843.

    CAS  Google Scholar 

  12. Franek, M., Kolar, V., Granatova, M., and Neverkova, Z.,J. Agric. Food Chem., 1994, vol. 42, pp. 1369–1374.

    Article  CAS  Google Scholar 

  13. Dzantiev, B.B., Zherdev, A.V., Romanenko, O.G., Titova, N.A., Trubacheva, Zh.N., Cherednikova, T.V., and Eremin, S.A.,Prikl. Biokhim. Mikrobiol., 1994, vol. 20, pp. 731–739.

    Google Scholar 

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Correspondence to I. A. Lubavina.

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Lubavina, I.A., Salomatina, I.S., Zinchenko, A.A. et al. A noninstrumental immunoassay based on colloidal dyes. Russ J Bioorg Chem 26, 207–212 (2000).

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Key words

  • competitive ELISA
  • colored colloidal dyes
  • 2,4-dichlorophenoxyacetic acid
  • dot-immunoassay
  • simazine