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Synthesis and properties of photolabile (caged) phosphotriester derivatives of dinucleoside phosphates


Dinucleoside phosphates that harbor phosphate groups transiently blocked (caged) byo-nitrobenzyl oro-nitroveratryl residues were synthesized. It was shown that the conditions of the UV-induced deprotection largely depend on the nature of the protective group. The phosphotriesters obtained were resistant toward snake venom phosphodiesterase and nucleases of the cellular extract. The synthesis of the dinucleoside phosphates containing a photolabile group preceeded the incorporation of the modified blocks into extended oligonucleotides by the phosphoramidite method.

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snake venom phosphodiesterase




tetrabutylammonium fluoride




reverse-phase chromatography. Prefix “d” for deoxyribooligonucleotides is omitted


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Correspondence to T. V. Abramova.

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Abramova, T.V., Leonetti, J.P., Vlassov, V.V. et al. Synthesis and properties of photolabile (caged) phosphotriester derivatives of dinucleoside phosphates. Russ J Bioorg Chem 26, 174–182 (2000).

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Key words

  • oligonucleotide derivatives
  • photolabile compounds