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Modification of blood group a expression in human pancreatic tumor cell lines by inhibitors ofN-glycan processing

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Summary

Pancreatic adenocarcinomas induced in Syrian hamsters byN-nitrosobis(2-oxopropyl)amine (BOP) treatment express blood group A (BGA) antigen, which was previously shown by this lab to be expressed on multiantennary asparagine (Asn)-linked glycans attached to membrane glycoproteins. To determine if a similar expression pattern was found in humans, three human pancreatic ductal adenocarcinoma cell lines (CD18, CD11, and Capan 1) from individuals of blood type A were analyzed and shown to express BGA antigen on membrane glycoproteins similar in molecular mass to those found in hamster tumor cells. The BGA antigen was located on Asn-linked oligosaccharides in all three human cell lines, as indicated by loss of activity after peptide:N-glycosidase F (PNGase F) treatment. Also, as shown previously in hamster pancreatic tumor cells, BGA expression at the surface of the human cell lines was blocked by growth of the cells in media containing deoxymannojirimycin (dMM), an inhibitor of mannosidase I. These results demonstrate that the BGA antigen is on Asn-linked glycans in human pancreatic adenocarcinoma cells and that these glycoproteins are processed similarly to the BGA glycoproteins in hamster pancreatic adenocarcinoma.

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Abbreviations

Asn:

asparagine

BGA:

blood group A

BGB:

blood group B

BOP:

N-nitrosobis(2-oxopropyl)amine

BSA:

bovine serum albumin

Con A:

Concanavalin A

dMM:

deoxymannojirimycin

Endo H:

Endoglycosidase H

L-PHA:

leukoagglutinating phytohemagglutinin

PBS:

phosphate-buffered saline

PNGase F:

peptide:N-glycosidase F

PMSF:

phenylmethylsulfonyl fluoride

PVDF:

polyvinylidine difluoride

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

Ser:

serine

SW:

swainsonine

Thr:

threonine

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Authors and Affiliations

  1. Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE

    Parviz M. Pour & William G. Chaney

  2. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 600 S. 42nd St., 68198-4525, Omaha, NE

    Courtney Schaffert & William G. Chaney

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  1. Courtney Schaffert
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  2. Parviz M. Pour
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  3. William G. Chaney
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Schaffert, C., Pour, P.M. & Chaney, W.G. Modification of blood group a expression in human pancreatic tumor cell lines by inhibitors ofN-glycan processing. Int J Pancreatol 21, 21–29 (1997). https://doi.org/10.1007/BF02785916

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  • DOI: https://doi.org/10.1007/BF02785916

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