Abstract
ASulfolobus solfataricus β-glycosidase expressed inSaccharomyces cerevisiae (Sβgly) was immobilized on chitosan activated with glutaraldehyde. The yield of immobilization was evaluated as 80%. Compared to the free β-glycosidase, the immobilized enzyme showed a similar pH optimum (pH = 7.0), the same increasing activity up to 80°C, improved thermostability, and no inhibition by glucose. Functional studies pointed out that the kinetic constant values for both enzymes were comparable. A bioreactor, assembled with the immobilized Sβgly, was used for glucose production. The values of cellobiose conversion increased on increasing residence time in the bioreactor, following a nonlinear trend. However, the highest glucose production/ min was obtained at a flow of 0.5 mL/min.
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Abbreviations
- ONPG:
-
ortho-nitrophenyl-β-D-galactopyrano-side
- Sβgly:
-
Sulfolobus solfataricus β-glycosidase
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D’Auria, S., Pellino, F., La Cara, F. et al. Immobilization on chitosan of a thermophilic βglycosidase expressed inSaccharomyces cerevisiae . Appl Biochem Biotechnol 61, 157–166 (1996). https://doi.org/10.1007/BF02785698
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DOI: https://doi.org/10.1007/BF02785698