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Intracellular distribution of selenium and the growth of mammary cells in culture

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Abstract

Retention of Se in CMT-13 cells increased with an increase in the concentration of selenite in the incubation medium, the duration of exposure, and the density of the culture. The enhanced toxicity of selenite coincided with a proportional increase in Se in both the cytoplasm and nucleus. About 90% of the accumulated Se was isolated with cytoplasmic macromolecules. Increased nuclear Se retention correlated with increased cytoplasmic Se retention. Greater quantities of cytosolic Se-containing proteins (74, 55, 41, 34, and 28 kDa) and a nuclear Se-containing protein (56 kDa) were detected as the quantity of Se within CMT-13 cells increased. These findings suggest that cellular retention and distribution of Se are determinanants of the degree of cellular growth inhibition caused by this trace element.

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Abbreviations

CMT-13:

canine mammary tumor cell line 13

GPX:

glutathione peroxidase

FBS:

fetal bovine serum

DTT:

DL-dithiothreitol

PMSF:

phenylmethylsulfonyl fluoride

TCA:

trichloroacetic acid

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

EDTA:

ethylenediamine tetraacetate

GSH:

glutathione

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Authors and Affiliations

  1. Department of Nutrition, Pennsylvania State University, 16802, University Park, PA

    Kyunghee Hwang & John A. Milner

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  1. Kyunghee Hwang
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  2. John A. Milner
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Hwang, K., Milner, J.A. Intracellular distribution of selenium and the growth of mammary cells in culture. Biol Trace Elem Res 51, 133–147 (1996). https://doi.org/10.1007/BF02785433

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  • DOI: https://doi.org/10.1007/BF02785433

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