Abstract
The authors examined the effects of manganese salts on the interaction of the AIDS-related pathogen,Cryptosporidium parvum, with human ileoadenocarcinoma (HCT-8) cells in vitro. Manganese (Mn) inhibited binding ofC. parvum sporozoite membrane antigens to intact, fixed HCT-8 cells in a dose-dependent fashion, whereas Ca++, Mg++, and Zn++ salts had no effect. Manganese was also found to affect sporozoite penetration of live HCT-8 cells, which resulted in a dose-dependent inhibition of parasite development. However, the levels of Mn++ needed in the live cell assays was approx 10-fold greater than in the fixed-cell assays. This inhibition of parasite development was not reversible when Ca++ or Mg++ were used as competitors. Oral supplementation of suckling mice infected withC. parvum with MnSO4 resulted in significant reductions and, in some cases, elimination of intestinally derived oocysts.
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Nesterenko, M.V., Woods, K.M. & Upton, S.J. Effects of managanese salts on the AIDS-related pathogen,Cryptosporidium parvum in vitro and in vivo. Biol Trace Elem Res 56, 243–253 (1997). https://doi.org/10.1007/BF02785297
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DOI: https://doi.org/10.1007/BF02785297