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Pancreatic phospholipase A2 in cerulein-induced acute pancreatitis in the rat

International journal of pancreatology volume 14, pages 261–267 (1993)Cite this article

Summary

We investigated the concentration of immunoreactive pancreatic phospholipase A2 (pan-PLA2) and the catalytic activity of phospholipase A2 (CA-PLA2) in plasma and pancreases of rats with cerulein-induced acute pancreatitis. Edematous pancreatitis with ascites and fat necroses in the abdominal cavity developed after 8 h infusion of cerulein (5 μg/kg/h). Large vacuoles were found in acinar cells and there were small areas of acinar cell necrosis. No pathological changes were seen in saline-infused control animals. Pancreatic PLA2 was localized by immunohistochemistry in pancreatic acinar cells in both groups of animals and in the proximal tubular cells of the kidney in cerulein-infused animals. The lungs and kidneys appeared normal by light microscopy in all animals. The pan-PLA2 values increased markedly, whereas the CA-PLA2 values did not change during the cerulein-infusion. The CA-PLA2 values in the homogenates of pancreatic tissue of cerulein-infused animals did not differ significantly from those of saline-infused controls. The results indicate that the CA-PLA2 in plasma is independent from the concentration of pan-PLA2 in cerulein-induced acute pancreatitis in rat.

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References

  1. Nevalainen TJ. The role of phospholipase A2 in acute pancreatitis.Scand J Gastroenterol 1980; 15: 641–650.

    PubMed  CAS  Article  Google Scholar 

  2. Nevalainen TJ. Phospholipase A2 in acute pancreatitis.Scand J Gastroenterol 1988; 23: 897–904.

    PubMed  Article  CAS  Google Scholar 

  3. Büchler M, Malfertheiner P, Schädlich H, Nevalainen TJ, Friess H, Beger HG. Role of phospholipase A2 in human acute pancreatitis.Gastroenterology 1989; 97: 1521–1526.

    PubMed  Google Scholar 

  4. Eskola JU, Nevalainen TJ, Kortesuo P. Immunoreactive pancreatic phospholipase A2 and catalytically active phospholipases A2 in serum from patients with acute pancreatitis.Clin Chem 1988; 34: 1052–1054.

    PubMed  CAS  Google Scholar 

  5. Nevalainen TJ, Eskola JU, Aho AJ, Havia VT, Lövgren TN-E, Näntö V. Immunoreactive Phospholipase A2 in serum in acute pancreatitis and pancreatic cancer.Clin Chem 1985; 31: 1116–1120.

    PubMed  CAS  Google Scholar 

  6. Uhl W, Büchler M, Nevalainen TJ, Deller A, Beger HG. Serum phospholipase A2 in patients with multiple injuries.J Trauma 1990; 30: 1285–1290.

    PubMed  Article  CAS  Google Scholar 

  7. Vadas P. Elevated plasma phospholipase A2 levels: Correlation with the hemodynamic and pulmonary changes in gram-negative septic shock.J Lab Clin Med 1984; 104: 873–881.

    PubMed  CAS  Google Scholar 

  8. Vadas P, Pruzanski W, Stefanski E, Sternby B, Mustard R, Bohnen J, Fraser I, Farewell V, Bombardier C. Pathogenesis of hypotension in septic shock: Correlation of circulating phospholipase A2 levels with circulatory collapse.Crit Care Med 1988; 16: 1–7.

    PubMed  Article  CAS  Google Scholar 

  9. Pruzanski W, Vadas P. Phospholipase A2—a mediator between proximal and distal effectors of inflammation.Immunol Today 1991; 12: 143–146.

    PubMed  CAS  Google Scholar 

  10. Kortesuo PT, Hietaranta AJ, Jämiä M, Hirsimäki P, Nevalainen TJ. Rat pancreatic phospholipase A2: Purification, localization and development of an immunoassay.Int J Pancreatol 1993; 13: 111–118.

    PubMed  CAS  Google Scholar 

  11. Schädlich HR, Büchler M, Beger HG. Improved method for the determination of phospholipase A2 catalytic activity concentration in human serum and ascites.J Clin Chem Clin Biochem 1987; 25: 505–509.

    PubMed  Google Scholar 

  12. Adler G, Hupp T, Kern HF. Course and spontaneous regression of acute pancreatitis in the rat.Virchows Arch (Pathol Anat) 1979; 382: 31–47.

    Article  CAS  Google Scholar 

  13. Lampel M, Kern HF. Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue.Virchows Arch (Pathol Anat) 1977; 373: 97–117.

    Article  CAS  Google Scholar 

  14. Watanabe O, Baccino FM, Steer ML, Meldolesi J. Supramaximal stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis.Am J Physiol 1984; 246: G457–467.

    PubMed  CAS  Google Scholar 

  15. Saluja A, Hashimoto S, Saluja M, Powers RE, Meldolesi J, Steer ML. Subcellular redistribution of lysosomal enzymes during caerulein-induced pancreatitis.Am J Physiol 1987; 253: G508–516.

    PubMed  CAS  Google Scholar 

  16. Willemer S, Adler G. Mechanism of acute pancreatitis. Cellular and subcellular events.Int J Pancreatol 1991; 9: 21–30.

    PubMed  CAS  Google Scholar 

  17. Hietaranta AJ, Laszik ZG, Aho HJ, Kortesuo PT, Nevalainen TJ. The role of phospholipase A2 in pancreatic acinar cell injury.Int J Pancreatol 1991; 8: 187–201.

    PubMed  Article  CAS  Google Scholar 

  18. Nagai H, Henrich H, Wünsch P-H, Fischbach W, Mössner J. Role of pancreatic enzymes and their substrates in autodigestion of the pancreas.Gastoenterology 1989;96: 838–847.

    CAS  Google Scholar 

  19. Sugimoto Y, Hayakawa T, Kondo T, Shibata T, Kishimoto W. Peritoneal absorption of pancreatic enzymes in bile-induced acute pancreatitis in dogs.J Gastroenterol Hepatol 1990; 5: 493–498.

    PubMed  Article  CAS  Google Scholar 

  20. Egdahl RH. Mechanism of blood enzyme changes following the production of experimental pancreatitis.Ann Surg 1958; 148: 389–400.

    PubMed  Article  CAS  Google Scholar 

  21. Howard JM, Smith AK, Peters JJ. Acute pancreatitis: pathways of enzymes into the blood stream.Surgery 1949; 26: 161–166.

    PubMed  CAS  Google Scholar 

  22. Lange JF, Beyaert PJ, van Vugt H, Tytgat GNJ, van Gool J. Pathways of enzyme transfer in sodium taurocholate— induced acute hemorrhagic pancreatitis.Digestion 1986; 35: 229–236.

    PubMed  CAS  Article  Google Scholar 

  23. Mayer AD, Airey M, Hodgson J, McMahon MJ. Enzyme transfer from pancreas to plasma during acute pancreatitis. The contribution of ascitic fluid and lymphatic drainage of the pancreas.Gut 1985; 26: 876–881.

    PubMed  Article  CAS  Google Scholar 

  24. Hietaranta AJ, Aho HJ, Grönroos JM, Hua ZY, Nevalainen TJ. Pancreatic phospholipase A2 in proximal tubules of rat kidney in experimental acute pancreatitis and after intravenous injection of the enzyme. Pancreas, 1992; 7: 326–333.

    PubMed  Article  CAS  Google Scholar 

  25. Kurtz VM, Ascherl R, Steger A, Wimmer B, Müller F, Blumel G. Untersuchungen zur systemichen Wirkung der Phospholipase A2.Klinsche Chemie Mitteilungen (Deutsche Gesellschaft für Klinische Chemie) 1986; 17: 210–220.

    Google Scholar 

  26. Rohr G, Scheele G. Fate of radioactive exocrine pancreatic proteins injected into the blood circulation of the rat. Tissue uptake and transepithelial excretion.Gastroenterology 1983; 85: 991–1002.

    PubMed  CAS  Google Scholar 

  27. Schröder T, Lempinen M, Kivilaakso E, Nikki P. Serum phospholipase A2 and pulmonary changes in acute fulminant pancreatitis.Resuscitation 1982; 10: 78–87.

    Article  Google Scholar 

  28. Morgan AP, Jenny ME, Haessler H. Phospholipids, acute pancreatitis and the lungs: Effect of lecithinase infusion on pulmonary surface activity in dogs.Ann Surg 1968; 167: 329–334.

    PubMed  Article  CAS  Google Scholar 

  29. Selig WM, Durham SK, Welton AF. Pulmonary responses to phospholipase A2 in the perfused guinea pig lung.J Appl Physiol 1989; 67: 2495–2503.

    PubMed  CAS  Google Scholar 

  30. Stömmer P, Steinmann U. Phospholipase A2 induced diffuse alveolar damage—effect of indomethacin and dexamethasone upon morphology and plasma—histamine level.Klin Wochenschr 1989; 67: 171–176.

    PubMed  Article  Google Scholar 

  31. Holm BA, Keicher L, Liu MY, Sokolowski J, Enhorning G. Inhibition of pulmonary surfactant function by phospholipases.J Appl Physiol 1991; 71: 317–321.

    PubMed  CAS  Google Scholar 

  32. Lankisch PG, Rahlf G, Koop H. Pulmonary complications in fatal acute hemorrhagic pancreatitis.Dig Dis Sci 1983; 28: 111–116.

    Article  Google Scholar 

  33. Maciver AG, Metcalfe IL, Possmayer F, Harding PGR, Passi RB. Alteration of surfactant chemistry in experimental hemorrhagic pancreatitis.J Surg Res 1977; 23: 311–314.

    PubMed  Article  CAS  Google Scholar 

  34. Feddersen CO, Willemer S, Karges W, Pückner A, Adler G, Wickert PV. Lung injury in acute experimental pancreatitis in rats. II. Functional studies.Int J Pancreatol 1991; 8: 323–331.

    PubMed  CAS  Google Scholar 

  35. Kelly DM, McEntee GP, McGeeney KF, Fitzpatrick JM. Pulmonary microvasculature in experimental acute haemorrhagic and oedematous pancreatitis.Br J Surg 1991; 78: 1064–1007.

    PubMed  Article  CAS  Google Scholar 

  36. Willemer S, Feddersen CO, Karges W, Adler G. Lung injury in acute experimental pancreatitis in rats. I. Morphological studies.Int J Pancreatol 1991; 8: 305–321.

    PubMed  CAS  Google Scholar 

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Affiliations

  1. Department of Pathology, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland

    Antti J. Hietaranta, Heikki J. Aho & Timo J. Nevalainen

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  1. Antti J. Hietaranta
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  2. Heikki J. Aho
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Hietaranta, A.J., Aho, H.J. & Nevalainen, T.J. Pancreatic phospholipase A2 in cerulein-induced acute pancreatitis in the rat. Int J Pancreatol 14, 261–267 (1993). https://doi.org/10.1007/BF02784935

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  • DOI: https://doi.org/10.1007/BF02784935

Key Words

  • Enzyme-immunoassay
  • immunohistochemistry
  • kidney tubular cells
  • pancreatic acinar cells
  • pancreatic phospholipase A2