Abstract
The effects of selenium on the activity of peroxidase (POD) of wheat seedling and its isozyme pattern were studied using a greenhouse hydroponic experiment. The results show that the activity of POD is increased in response to higher Se concentration (approx 5.0 mg/L) in culture medium. The electrophoretic pattern of the POD isozyme was altered by growth of the wheat in a selenium medium. Se could incorporate into some POD isozymes during either seed germination or the seedling growth period. There is a dose-responsive incorporation of selenium in isozyme of POD and selenium content in the isozyme increase along with the increase of selenium concentration in the medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
C.A. Cameron, Preliminary note on the absorption of selenium by plants,Sci. Proc. Roy. Dublin Soc. 2, 231–233 (1980).
T. A. Brown and A. Shrift, Selenium: toxicity and tolerance in higher plant,Biol. Rev. 57, 59–84 (1982).
S. H. Diehn, W. Burkhart, and J. S. Graham, Purification and partial amino acid sequence of a wound-inducible, developmentally regulated anionic peroxidase form,Biochem. Biophys. Res. Commun. 195, 928–934 (1993).
Z. J. Zhang F. Fang, and Q. F. Lu, Effect of mercury on the growth and isozyme of peroxidase wheat seedlings,Acta Sci. Circumstantiae Sinica 9, 371–374 (1989).
B. J. Davis, Disc electrophoresis: method and application to human serum protein,Ann NY Acad. Sci. 121, 404 (1964).
Z. C. Zhu, Determination of activity of peroxidase,Adv. Biochem. Biophys. 6, 80–83 (1985).
Z. J. Wang and J. F. Sun, Selenium determination at ppt level using a HPLC-FLD system,Acta Sci. Circumstantiae Sinica 8, 121–124 (1988).
J. X. Sun, Y. Q. Wang, Y. Xu, Z. J. Wang, and A. Peng, Determination of selenium in the band of peroxidase isozyme of wheat by neutron activation techniques,Chin. Sci. Bull. 42, 1770–1773 (1997).
K. X. Huang, Study on selenium-containing bio-macromolecule in plant, Ph.D. thesis, Hua-Zhong Polytechnical University, Wuhan, China, pp. 27–29 (1995).
A. Ishida, K. Ono, and T. Matsusaka, Cell wall-associated peroxidase in cultured cells of livewort,Marchantis polymorpha L. changes of peroxidase level and its location in the cell wall,Plant Cell Rep. 5, 54 (1985).
P. H. R. Van Hasselt, Photo-oxidatiion of leaf pigment in cucumber leaf discs during chilling,Acta Bot. Neerl. 21, 539 (1972).
Z. J. Wang, Y. Xu and A. Peng, Influences of fulvic acid on bioavailability and toxicity of selenite for wheat seedling and growth,Biol. Trace Element Res. 55, 147–162 (1996).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peng, A., Xu, Y. & Wang, Z.J. Study of the incorporation of selenium into peroxidase isozyme of wheat seedling. Biol Trace Elem Res 70, 117–125 (1999). https://doi.org/10.1007/BF02783853
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02783853