Abstract
Comparison of the clastogenic effects of antimony and bismuth used as trioxides, when administered orally by gavaging to laboratory bred male mice, showed that the former was more strongly clastogenic than the latter. Three doses of each chemical (400, 666.67, and 1000 mg/kg body wt), corresponding to 1/50, 1/30, and 1/20 of oral LD50 of antimony trioxide, were fed daily to sets of mice up to 21 d. Animals were sacrificed on day 7, 14, and 21 of the experiment. Chromosomal aberrations and mitotic index were studied from bone marrow cells following a colchicine-air drying Giemsa schedule. The frequencies of chromosomal aberrations induced by both chemicals were directly proportional to the dose used and the duration of exposure, indicating their cumulative effects on the organism. The highest dose of antimony, given for the longest period was, however, lethal. Effects on germ cells, as shown by screening for sperm head abnormalities, were not significant.
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References
B. A. Fowler, and P. L. Goering,Antimony in Metals and Their Compounds in the Environment, E. Merian, ed.,743, VCH Publication, Weinheim (1991).
MAK, Maximum Concentrations at the Workplace, Report No. XXIII, DFG, VCH Publication, Weinheim (1987).
G. Emmerling, K. H. Schaller and H. ValentinZentralbl Arbeitsmet 36, 258 (1986).
D. W. Thomas,Metals and Their Compounds in the Environment, E. Merian, ed.,789, VCH Publication, Weinheim (1991).
B. Venugopal and T. D. Luckey,Metal Toxicity in Mammals 2, New York, Plenum (1978).
Merck Index 10th edition, M. Windholz, ed., Published by Merck and Co., Inc. Rahway, New Jersey., USA (1983).
R. J. Preston, B. D. Dean, S. Galloway, H. Holden, A. F. McFee, and M. ShelbyMutat. Res. 189, 157 (1987).
J. Sakamoto and K. HashimotoArch of Toxicology,62, 54 (1988).
A. J. Wyrobek, G. Watchmaker, and L. GordonHandbook of Mutagenicity Test Procedures (B. J. Kilbey, M. Legator, W. Nichols, and C. Ramel, eds.) Elsevier, Amsterdam. 739 (1984).
A. K. Sharma and A. Sharma,Chromosome Techniques Theory and Practice 3rd ed. Butterworth, London (1980).
R. R. Tice, R. Boucher, C. A. Luke, and M. D. Shelby,Environ. Mutagen. 9, 235 (1987).
B. H. Margolin, M. A. Resnick, J. Y. Rimpo, P. Archer, S. M. Galloway, A. D. Bloom, and E. Zeiger,Environ. Mol. Mutagen. 8, 183 (1986).
R. A. Fisher and F. Yates,Statistical Tables for Biological, Agricultural and Medical Research, 6th ed. Oliver, Boyd Edinburgh 1963).
R. R. Sokal and F. J. RohltBiometry, Freeman, San Francisco, CA (1981).
D. B. Duncan,Biometrics,11, 1 (1955).
H. L. Harter,Biometrics,16, 671 (1960).
T. C. Hsu,Cytogenetic Assays of Environmental Mutagens (ed 1, NJ Allanheld Osmun (1982).
International Commission for Protection against Environmental Mutagens and Carcinogens, Report of Committee I, Mutation Res.114, 120 (1983).
World Health Organization, Environmental Health Criteria 51, Guide to short term tests for detecting mutagenic and carcinogenic, chemicals, WHO, Geneva, 100 (1985).
W. Bruce and J. A. Heddle,Can. J. Genet. Cytol. 21, 319 (1979).
J. C. Topham,Mutation Res.,74, 379 (1980).
A. J. Wyrobek, W. R. BruceProc. Natl. Acad. Sci. (USA) 72, 4425 (1975).
A. J. Wyrobek, L. A. Gordon, J. K. Burkhart, M. W. Francis, R. W. Kapp, G. Letz, H. V. Malling, J. C. Topham, and M. D. Whorton,Mutation Res. 115, 1 (1983).
N. Gurnani, A. Sharma, and G. Talukder,Biology of Metals, Germany (in press) (1991).
W. J. Hayes, Jr., Tests for detecting and measuring long term toxicity, inEssays in Toxicology W. J. Hayes, ed., Jr. Vol. III, Academic Press, New York.
N. Ainsworth and J. A. Cooke,Environ, Pollut.65, 79 (1990).
D. Chaleil and J. P. RegnaultTrace Elem, Med. 2, 77 (1985).
T. Stolman and C. P. Stewart, eds., Metallic Poisons, inToxicology Mechanism and Analytical Methods, Vol. I, Academic Press, New York and London (1960).
J. Huang and W. Xiangpu,Zhongguo Yaolixue Yu Dulixue Zazhi 2, 288 (1988).
F. Rossi, R. Acampora, C. Vacca, and E. Marmo,Teratog. Carcinog. Mutagen.7, 491 (1987).
A. Martindale and J. H. Turner,J. Nucl. Med. 21, 1035 (1980).
K. Nagai and S. YanagimotoJpn. J. Nucl. Med. 17, 583 (1980).
O. P. D. Noronha and A. B. SewatkarNucl. Med. Biol. 13, 67 (1986).
P. C. Mangal and J. S. Sohal,Indian J. Exp. Biol. 16, 685 (1978).
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Gurnani, N., Sharma, A. & Talukder, G. Comparison of clastogenic effects of antimony and bismuth as trioxides on mice in vivo. Biol Trace Elem Res 37, 281–292 (1993). https://doi.org/10.1007/BF02783802
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DOI: https://doi.org/10.1007/BF02783802