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Determination of isotope ratios in human tissues enriched with zinc stable isotope tracers using inductively coupled plasma-mass spectrometry (ICP-MS)

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Abstract

Enriched fecal and urine samples were prepared using ion-exchange column chromatography for analysis by Inductively Coupled and Fast Atom Bombardment Mass spectrometry (ICP-MS, FAB-MS) to compare precision between methods. Unenriched samples of human milk, feces, and whole blood were prepared similarly to monitor instrumental precision and analytical error. A least squares fit of the ICP-MS results vs the FAB-MS for70Zn/64Zn gave a slope of 0.98, with a relative standard deviation (RSD) of only 0.7%. The results for68Zn/64Zn gave a slope of 0.82, with a RSD of 14%. For unenriched tissues, all potential interferences were removed by the preparation procedure with no significant differences between preparation for isotope ratios of70Zn/64Zn,68Zn/64Zn,67Zn/64Zn, and66Zn/64Zn. Poisson counting statistics are a major contribution to the total analytical error indicating the usefulness of this procedure for enrichment studies.

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Authors and Affiliations

  1. Department of Biochemistry, Memorial University of Newfoundland, A1B 3X9, St. John's, Newfoundland, Canada

    James K. Friel

  2. Department of Earth Sciences, Memorial University of Newfoundland, A1B 3X9, St. John's, Newfoundland, Canada

    Henry P. Longerich & Simon E. Jackson

  3. Center for Earth Resources Research, Memorial University of Newfoundland, A1B 3X9, St. John's, Newfoundland, Canada

    James K. Friel, Henry P. Longerich & Simon E. Jackson

Authors
  1. James K. Friel
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  2. Henry P. Longerich
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  3. Simon E. Jackson
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Friel, J.K., Longerich, H.P. & Jackson, S.E. Determination of isotope ratios in human tissues enriched with zinc stable isotope tracers using inductively coupled plasma-mass spectrometry (ICP-MS). Biol Trace Elem Res 37, 123–136 (1993). https://doi.org/10.1007/BF02783788

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  • DOI: https://doi.org/10.1007/BF02783788

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