Abstract
Interactions between manganese (Mn) deficiency and streptozotocin (STZ)-diabetes with respect to tissue antioxidant status were investigated in male, Sprague-Dawley rats. All rats were fed either a Mn-deficient (1 ppm) or a Mn-sufficient (45 ppm) diet for 8 wk. Diabetes was then induced by tail-vein injection of STZ (60 mg/kg body weight), after which the rats were kept for an additional 4 or 8 wk. The control groups comprised rats not injected with STZ and fed either Mn-deficient or Mn-sufficient diets for a total of 12 wk.
The Mn-deficient diet decreased the activities of manganese superoxide dismutase (MnSOD) in kidney and heart, and of copperzinc superoxide dismutase (CuZnSOD) in kidney, in the non-diabetic animals. In the diabetic rats, the Mn-deficient diet induced more pronounced decreases in activities of these same enzymes, and also increased liver MnSOD activity. Plasma and hepatic vitamin E levels increased progressively with the duration of diabetes, independent of dietary Mn intake. Lipid peroxidation, as measured by H2O2-induced production of thiobarbituric acid reactive substances in erythrocytes, also increased, concomitant with decreased liver and kidney glutathione (GSH) levels. These findings demonstrate for the first time an interactive effective between Mn deficiency and STZ-diabetes, resulting in amplification of tissue antioxidant changes seen with either Mn deficiency or STZ-diabetes alone. This effect of Mn deprivation in experimental diabetes suggests a physiological role for Mn as an antioxidant nutrient.
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References
D. I. Paynter,J. Nutr. 110, 437 (1980).
D. I. Paynter,Biol. Trace Elem. Res. 2, 121 (1980).
D. L. Baly, D. L. Curry, C. L. Keen, and L. S. Hurley,J. Nutr. 114, 1438 (1984).
D. L. Baly, D. L. Curry, C. L. Keen, and L. S. Hurley,Endocrinology 116, 1734 (1985).
M. Nishida, H. Sakurai, J. Kawada, M. Koyama, and J. Takada,Naturwissenschaften 76, 220 (1989).
M. L. Failla,Biol. Trace Elem. Res. 5, 275 (1983).
R. B. Rucker, J. Uriu-Hare, D. Tinker, and C. L. Keen,FASEB. J. 5, A583 (1991).
J. S. Bond, M. L. Failla, and D. F. Unger,J. Biol. Chem. 258, 8004 (1983).
S. A. Spittle and M. L. Failla,Biol. Trace Elem. Res. 5, 489 (1983).
S. A. Wohaieb and D. V. Godin,Diabetes 36, 1014 (1987).
D. Loven, L. Oberley, H. Schedl, and H. Wilson inOxy Radicals and Their Scavenger Systems, Vol II: Cellular and Medical Aspects, R. A. Greenwald and G. Cohen, eds., Elsevier Science Publishing Co., Inc., New York, 1983, pp. 17–21.
D. Loven, H. Schedl, H. Wilson, T. T. Diaabees, L. D. Stegnik, M. Diekus, and L. Oberley,Diabetes 35, 503 (1986).
G. de Rosa, C. L. Keen, R. M. Leach, and L. S. Hurley,J. Nutr. 110, 795 (1980).
S. A. Wohaieb and D. V. Godin,Diabetes 36, 169 (1987).
K. H. Winterhalter,Methods Enzymol. 76, 732 (1981).
M. S. Clegg, C. L. Keen, B. Lönnerdal, and L. S. Hurley,Biol. Trace Elem. Res. 3, 237 (1981).
H. P. Misra and I. Fridovich,J. Biol. Chem. 247, 3170 (1972).
M. Sun and S. Zigman,Anal. Biochem. 90, 81 (1978).
L. Flohé, inCRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol III., J. Miquel, A. T. Quintanilha, and H. Weber, eds., CRC Press, Boca Raton, FL, 1989, pp. 281–286.
E. Racker,Methods Enzymol. 2, 722 (1955).
H. Aëbi, inMethods of Enzymatic Analysis, 2nd Ed., H. U. Bergmeyer, ed., Academic Press, New York, 1974, pp. 673–684.
I. D. Desai, inCRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. III., J. Miquel, A. T. Quintanilha, and H. Weber, eds., CRC Press, Boca Raton, FL, 1989, pp. 247–252.
J. K. Lang, K. Gohil, and L. Packer,Anal. Biochem. 157, 106 (1986).
M. S. Moron, J. W. Depierre, and B. Mannervik,Biochim. Biophys. Acta 582, 67 (1979).
M. J. Norusis,SPSS/PC+ Statistics 4.0 ™ for the IBM PC/XT/AT and PS/2, SPSS, Inc., Chicago, IL, 1986, pp. B25-B48.
C. D. Davis, D. M. Ney, and J. L. Greger,J. Nutr. 120, 507 (1990).
J. Y. Uriu-Hare, J. S. Stern, and C. L. Keen,Diabetes 38, 1282 (1989).
A. D. Mooradian and J. E. Morley,Am. J. Clin. Nutr. 45, 877 (1987).
G. H. W. Wong, J. H. Elwell, L. W. Oberley, and D. V. Goeddel,Cell 58, 923 (1989).
N. Nath, S. N. Chari, and A. B. Rathi,Diabetes 33, 586 (1984).
C. L. Keen, T. Tamura, B. Lönnerdal, L. S. Hurley, and C. H. Halsted,Am. J. Clin. Nutr. 41, 929 (1985).
S. Zidenberg-Cherr, L. S. Hurley, B. Lönnerdal, and C. L. Keen,J. Nutr. 115, 460 (1985).
M. Brandt and V. L. Schramm, inManganese in Metabolism and Enzyme Function, V. L. Schramm and F. C. Wedler, eds., Academic Press, Orlando, FL, 1986, pp. 3–16.
Y. Higuchi,Acta Med. Okayama 36, 165 (1982).
K. A. Pritchard, Jr., S. T. Patel, C. W. Karpen, H. A. I. Newman, and R. V. Panganamala,Diabetes 35, 278 (1986).
W. A. Behrens, F. W. Scott, and R. Madere,Life Sci. 35, 199 (1984).
C. K. Chow,Am. J. Clin. Nutr. 32, 1066 (1979).
D. W. Morel and G. M. Chisolm,J. Lipid Res. 30, 1827 (1989).
S. P. Wolff, inDiabetic Complications. Scientific and Clinical Aspects, M. J. C. Crabbe, ed., Churchill Livingstone, Edinburgh/London/Melbourne/New York, 1987, pp. 167–220.
D. V. Godin, S. A. Wohaieb, M. E. Garnett, and A. D. Goumeniouk,Mol. Cell Biochem. 84, 223 (1988).
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Thompson, K.H., Godin, D.V. & Lee, M. Tissue antioxidant status in streptozotocin-induced diabetes in rats. Biol Trace Elem Res 35, 213–224 (1992). https://doi.org/10.1007/BF02783767
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DOI: https://doi.org/10.1007/BF02783767