Abstract
The first development of an α-face-specific radioimmunoassay for vitamin B12 is described. Sheep, fed a cobalt-deficient diet, and immunized with a conjugate between Co-β carboxypropyl cobalamin and keyhole limpet hemocyanin, were used to produce antisera. The antisera crossreacted with Co-β derivatives of vitamin B12, but did not crossreact with the α-face vitamin B12 analog cobinamide. The antisera were used to develop a sensitive and reproducible radioimmunoassay that was free from contamination with the nonspecific vitamin B12 binding protein, R-protein. Both the radioimmunoassay and measurements of plasma concentrations of methylmalonic acid were applied to the diagnosis of cobalt/vitamin B12 deficiency in sheep. The assay correlated well with a commercially available radioassay and did not falsely detect normal vitamin B12 concentration in plasma samples containing elevated concentrations of methylmalonic acid.
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Kennedy, D.G., Blanchflower, W.J., Young, P.B. et al. Development and application of an α-face-specific radioimmunoassay for vitamin B12 . Biol Trace Elem Res 35, 153–166 (1992). https://doi.org/10.1007/BF02783727
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DOI: https://doi.org/10.1007/BF02783727