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Zinc uptake by blood cells of rats in zinc deficiency and inflammation

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Abstract

In zinc deficiency, the function of leukocytes is impaired. However, the results of studies on the zinc concentration of blood cells in zinc deficiency are conflicting, probably in part because of technical and analytical problems. The aim of this study was to investigate, under standard conditions, the uptake of65Zn-labeled zinc by blood cells, taken from zinc-deficient rats and from rats in which an inflammation is induced. In both conditions, the serum zinc concentration is reduced. In clinical practice, this makes it difficult to determine whether the decrease in serum zinc is the result of a real or an apparent zinc deficiency. In stress, like an inflammatory disease, the decrease of zinc reflects an apparent zinc deficiency because of redistribution of serum zinc into the liver and because of decrease in serum albumin concentration. Over 70% of the serum zinc is bound to albumin. Blood cells from zinc-deficient and control rats were isolated using a discontinuous Percoll gradient and incubated under nearly physiological conditions in a65Zn-containing medium. A significant increase in the in vitro uptake of65Zn-labeled zinc by the blood cells of zinc-deficient rats was seen: erythrocytes 1.3, mononuclear cells 2.0, and polymorphonuclear cells 2.6 times the control values. During inflammation, no change in65Zn-labeled zinc uptake by erythrocytes and mononuclear cells was demonstrated after 2 d, although the serum zinc and albumin concentrations were decreased, but a small but significant increase in zinc uptake by polymorphonuclear cells was observed. This study of65Zn uptake in vitro under standard conditions may prove of value for distinguishing in patients real zinc deficiency from apparent zinc deficiency owing to, e.g., stress, although additional experiments should be performed.

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Authors and Affiliations

  1. Department of Medicine, Division of Gastrointestinal and Liver Diseases, University Hospital Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, the Netherlands

    Ton H. J. Naber & Jan H. M. van Tongeren

  2. Department of Nuclear Medicine, University Hospital Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, the Netherlands

    Wim J. M. van den Broek

  3. Department of Radiochemistry, Interfaculty Reactor Institute, Technical University, Mekelweg 15, 2629 JB, Delft, the Netherlands

    Cornelis J. A. van den Hamer

Authors
  1. Ton H. J. Naber
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  2. Cornelis J. A. van den Hamer
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  3. Wim J. M. van den Broek
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  4. Jan H. M. van Tongeren
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Additional information

A part of this study has been presented at the Meeting of The American Gastroenterological Association on May 12–18, 1990, San Antonio, TX, and has been published in abstract inGastroenterology 98 suppl., A423.

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Naber, T.H.J., van den Hamer, C.J.A., van den Broek, W.J.M. et al. Zinc uptake by blood cells of rats in zinc deficiency and inflammation. Biol Trace Elem Res 35, 137–152 (1992). https://doi.org/10.1007/BF02783726

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  • DOI: https://doi.org/10.1007/BF02783726

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