Abstract
The metabolism of selenite, selenocysteine (SeCys), and selenomethionine (SeMet) was studied in three human lymphoblast cell lines with defects in the transsulfuration pathway and in control cells without this defect. There were very little differences in the induction of glutathione peroxidase (GPX) activity by selenite and SeCys among these cells. However, markedly higher levels of SeMet were required to induce GPX activity in transsulfuration defective cells than in control cells. Surprisingly, the addition of pyridoxal phosphate (PLP) to the media resulted in elevated GPX activity in all cells regardless of the chemical form of Se used. There is no explanation for this effect of PLP, but it is not through direct reaction with GPX or on the alteration of sulfhydryl groups.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
S. T. Omaye and A. L. Tappel,J. Nutr. 104, 747 (1974).
F. Pan and H. Tarver,Arch. Biochem. Biophys. 119, 429 (1967).
E. Nobuyoshi, T. Nakamura, H. Tanaka, T. Suzuki, Y. Morino, and K. Soda,Biochemistry 20, 4492 (1981).
K. Yasumoto, K. Iwami, and M. Yoshida,J. Nutr. 109, 760 (1979).
R. A. Sunde, W. K. Sonenburg, G. E. Gutzke, and W. G. Hoekstra,TEMA-4, (J. McHowell, J. W. Gawthorne, and C. L. White, eds.,Aust. Acad. Sci., Canberra. 4, 165 (1981).
D. M. Greenberg,Metabolic Pathways, 3rd ed., vol. 7 Academic Press, New York, 1975, p. 505.
L. A. Smolin, and N. J. Benevenga,J. Nutr. 112, 1264 (1982).
D. B. Hope,Biochem. J. 66, 486 (1957).
M. A. Beilstein, and P. D. Whanger,J. Nutr. 119, 1962 (1989).
M. A. Beilstein, W. A. Gahl, and P. D. Whanger,TEMA-6, L. S. Hurley, C. L. Keen, B. Lonnerdal, and R. B. Rucker, eds.,6, 323 (1989).
G. F. Moore, R. E. Gerner, and H. A. Franklin,Am. Med. Assoc. 199, 519 (1967).
M. A. Beilstein, P. D. Whanger, and L. Wong, inSelenium in Biology and Medicine, Part A, Van Nostrand Reinholt Co., New York, 1987, p. 197.
M. W. Brown, and J. M. Watkinson,Analyt. Chim. Acta 89, 29 (1977).
D. E. Paglia, and W. N. Valentine,J. Lab. Clin. Med. 70, 158 (1967).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall,J. Biol. Chem. 193, 265 (1951).
D. J. Finney, inStatistical Method in Biological Assay, Hafner Publishing Co., New York, 1952, p. 454.
W. H. Allaway, and E. E. Cary,Anlyt. Chem. 36, 1359 (1964).
J. Neter, W. Wasserman, and W. H. Hunter. Richard D. Irwin, Inc., Homewood, IL, 1983.
S. H. Mudd, and H. L. Levy, inThe Metabolic Basis of Inherited Disease. McGraw-Hill, New York, 1983, p. 522.
K. P. McConnell, and G. J. Cho,Am. J. Physiol. 208, 1191 (1965).
M. A. Beilstein, and P. D. Whanger,J. Inorg. Biochem. 29, 137 (1987).
P. D. Whanger, and M. A. Beilstein,FASEB J. 2, A1439 (1988).
M. A. Grillo,Enzymologia 34, 7 (1967).
N. Esaki, N. Karai, T. Nakamura, H. Tanaka, and K. Soda,Arch. Biochem. Biophys. 238, 418 (1985).
J. T. Deagen, J. A. Butler, M. A. Beilstein, and P. D. Whanger,J. Nutr. 117, 91 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beilstein, M.A., Whanger, P.D. Selenium metabolism and glutathione peroxidase activity in cultured human lymphoblasts. Biol Trace Elem Res 35, 105–118 (1992). https://doi.org/10.1007/BF02783723
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02783723