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Cytotoxic and fluorescent assays for thymocyte subpopulations differing in surface thy-1 level

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Abstract

A number of analytical techniques for distinguishing and separating the “high θ” and “low θ” subpopulations of mouse thymocytes have been compared. A differential cytotoxic assay was compared to a quantitative immunofluorescent assay on individual cells using flow cytofluorometry and cell sorting. Conventional anti-Thy-1 antisera were compared with a monoclonal IgM anti-Thy-1. The monoclonal reagent greatly improved both types of assay, eliminated a number of artifacts and allowed either procedure to be used to give a clear distinction, based on Thy-1 level, between the two subpopulations. The distribution of Thy-1 on thymocytes is bimodal, rather than continuous. These separate “high θ” and “low θ” categories each includes a population of dividing cells.

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Authors and Affiliations

  1. The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

    Ken Shortman, D. Scott Linthicum, Frank L. Battye & Irving Goldschneider (American Cancer Society Eleanor Roosevelt-International Cancer Fellow)

  2. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille, France

    Ken Shortman, Andre Liabeuf & Pierre Golstein

  3. Department of Pathology, University of Connecticut Health Center, 06032, Farmington, CT., U.S.A.

    Irving Goldschneider (American Cancer Society Eleanor Roosevelt-International Cancer Fellow)

  4. Department of Zoology, University College, London, England

    Edward A. Clark & Phil Lake

Authors
  1. Ken Shortman
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  2. D. Scott Linthicum
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  3. Frank L. Battye
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  4. Irving Goldschneider
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  5. Andre Liabeuf
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  6. Pierre Golstein
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  7. Edward A. Clark
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  8. Phil Lake
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Shortman, K., Linthicum, D.S., Battye, F.L. et al. Cytotoxic and fluorescent assays for thymocyte subpopulations differing in surface thy-1 level. Cell Biochem Biophys 1, 255–270 (1979). https://doi.org/10.1007/BF02783667

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  • DOI: https://doi.org/10.1007/BF02783667

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