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Subclassification of murine T cells by computerized microphotometric analysis

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Abstract

Splenocytes separated by physical means and classified as T cells by immunologic tests and computerized microphotometric analysis are differentiated into subgroups by analysis of the distribution patterns of Feulgen-positive nuclear DNA. In like fashion T cells obtained as purified preparations after separation on a nylon column, and accepted as T cells by micromorphometric analysis were subjected to further computerized morphometric analysis of nuclear DNA to form subgroups of cells. In each case, the number and composition of the detected subgroups were consistent. The classification does not appear to reflect any obvious phases of the cell cycle and is not dependent upon the sex and strain of mice from which the cells were obtained.

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Authors and Affiliations

  1. Department of Microbiology, University of Arizona, 85721, Tucson, Arizona

    George B. Olson & Peter H. Bartels

  2. Department of Pathology, University of New Mexico, School of Medicine, 87131, Albuquerque, New Mexico

    Robert E. Anderson

Authors
  1. George B. Olson
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  2. Peter H. Bartels
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  3. Robert E. Anderson
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Olson, G.B., Bartels, P.H. & Anderson, R.E. Subclassification of murine T cells by computerized microphotometric analysis. Cell Biochem Biophys 1, 243–254 (1979). https://doi.org/10.1007/BF02783666

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  • DOI: https://doi.org/10.1007/BF02783666

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