Abstract
Splenocytes and column-separated T cells are differentiated into subpopulations of T and B cells on the basis of computer-assisted morphometric analysis of Feulgen-positive nuclear DNA. Differentiation is based upon the analysis of computable image information related to DNA distribution patterns. The technique at the present time does not allow immunofluorescent and morphometric measurements to be made on a given cell. However, the differentiation obtained by using descriptors proven capable of detecting pure populations of T and B cells shows excellent agreement with the differentiation obtained by immunofluorescence analysis. The descriptors and decision rules used in the discrimination among splenocytes are reproducible from one experiment to another and remain valid for the differentiation of lymphocytes from animals of different sex and strain.
Similar content being viewed by others
References
Greaves, M. F., and Hogg, N. M. (1971),Prog. Immunol. 14, 111.
Pierce, C. W., Asofsky, R., and Solliday, S. M. (1973),Fed. Proc. 32, 41.
Rief, A. E., and Allen, J. M. V. (1964),J. Exptl. Med. 120, 413.
Yan Yu, D. T. (1975),J. Immunol.,115, 91.
Vassar, P. S., Levy, E. M., and Brooks, D. E. (1976),Cell. Immunol. 21, 257.
Cantor, H., Simpson, E., Sato, U. L., and Herzenberg, L. A. (1975),Cell. Immunol. 15, 180.
Olson, G. B., Anderson, R. E., and Bartels, P. H. (1974),Cell. Immunol. 13, 347.
Bartels, P. H., Olson, G. B., Anderson, R. E., and Wied, G. L. (1975),Acta. Cytol. 19, 53.
Anderson, R. E., Olson, G. B., Howarth, J. L., and Bartels, P. H. (1975),Amer. J. Path. 80, 21.
Bartels, P. H., Bahr, G., Jeter, W. S., Olson, G. B., and Wied, G. L. (1974),J. Histochem. Cytochem. 22, 69.
Olson, G. B., Marie, K., and Bartels, P. H. (1977),Fed. Proc. 36, 1084.
Bartels, P. H., Bahr, G. P., Bibbo, M., and Wied, G. L. (1972),J. Histochem. Cytochem. 20, 239.
Olson, G. B., Wied, G. L., and Bartels, P. H. (1973),Acta Cytol. 17, 454.
Olson, G. B., and Bartels, P. H. (1974).Fed. Proc. 33, 733.
Anderson, R. E., Olson, G. B., Howarth, J. L., Troup, G. M., and Bartels, P. H. (1977),J. Immunol. 118, 1191.
Bianco, C., Patrick, R., and Nussenzweig, V. (1970),J. Exptl. Med. 132, 702.
Prewitt, J. M. S., and Mendelsohn, M. L. (1966),Ann. N.Y. Acad. Sci. 128, 1035.
Wied, G. L., Bartels, P. H., Bahr, G. F., and Oldfield, D. G. (1968),Acta Cytol. 12, 180.
Bartels, P. H., Bahr, G. F., and Wied, G. L. (1969),Acta Cytol. 13, 210.
Pressman, N. J. (1976),J. Histochem. Cytochem. 24, 138.
Vidal, B., Schleuter, G., Moore, G. W. (1973),Acta Cytol. 17, 510.
Bradley, J. V. (1968), InDistribution-free Statistical Tests, Prentice Hall, Englewood Cliffs, N.J., pp. 201–219.
Bartels, P. H., Olson, G. B., Jeter, W. S., and Wied, G. L. (1974),Acta Cytol. 18, 376.
Cooley, W. S. and Lohnes, P. R. (1962), InMultivariate Data Analysis, Wiley, N. Y., pp. 223–241.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olson, G.B., Bartels, P.H. & Anderson, R.E. Characterization of murine T and B cells by computerized microphotometric analysis. Cell Biochem Biophys 1, 229–242 (1979). https://doi.org/10.1007/BF02783665
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02783665