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One-step electroblotting of 2- to 100-kDa proteins onto a PVDF membrane

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Abstract

This article describes a method for electroblotting peptides and small proteins (< 100 kDa) from tricine gels onto a PVDF membrane. The major potential problem with these types of procedures is that proteins tend to stay in the gel under conditions where peptides are effectively eluted. The suggested protocol allows the complete transfer and binding of proteins and peptides in the range of 2–97 kDa.

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Authors and Affiliations

  1. Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain

    Juan Parrado, Antonio Ayala & Alberto Machado

Authors
  1. Juan Parrado
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  2. Antonio Ayala
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  3. Alberto Machado
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Parrado, J., Ayala, A. & Machado, A. One-step electroblotting of 2- to 100-kDa proteins onto a PVDF membrane. Appl Biochem Biotechnol 53, 285–292 (1995). https://doi.org/10.1007/BF02783502

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  • DOI: https://doi.org/10.1007/BF02783502

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