Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 229–244 | Cite as

Interesterification of butter fat by lipase fromRhizopus niveus in cosurfactant-free microemulsion system

  • S. Kermasha
  • M. Safari
  • M. Goetghebeur
Original Articles


Butter fat was interesterified in a cosurfactant-free microemulsion system containing nonionic and ionic surfactants, using commercial lipase obtained fromRhizopus niveus, at different concentrations of surfactant mixtures and hydrophilic-lipophilic balance (HLB) values. The results indicated that the interesterification yield (IY) of lipasecatalyzed interesterified butter fat reached its maximum in the microemulsion system prepared with the surfactant mixture of HLB value of 9, followed by that of HLB value of 10. In addition, increasing concentrations of surfactant mixtures, from 3 to 6 mM, resulted by an increase in the IY. The interesterification of butter fat in the microemulsion prepared with 3 mM of surfactant mixture of HLB value of 10 showed a minimum hydrolytic activity. The results showed that the interesterified selected triacylglycerol molecules were enriched with the hypocholesterolemic fatty acid C18:l, originally located onsn-1,3 positions, on theirsn-2 positions; this fatty acid was favorably interchanged with the hypercholesterolemic fatty acid C16:0, originally located onsn-2 position. The results also indicated that the use of 6 mM of surfactant mixtures increased the acyl exchange reaction toward the long-chain saturated fatty acid C16:0 on the sn-2 position of triacylglycerol molecules.

Index Entries

Interesterification lipase butter fat cosurfactantfree microemulsion system 


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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • S. Kermasha
    • 1
  • M. Safari
    • 1
  • M. Goetghebeur
    • 1
  1. 1.Department of Food Science and Agricultural ChemistryMcGill UniversityQu’ebecCanada

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