Summary
The polymerase chain reaction (PCR) followed by Southern blotting was used to examine the presence of hepatitis B virus (HBV) DNA in non-cancerous liver tissue specimens from 22 Japanese hepatocellular carcinoma (HCC) patients, who were negative for HBV surface antigen (HBsAg). By Southern blot analysis, HBV DNA was negative in all 22 patients, but it was detected by the PCR in 8 of the 15 patients who were positive for antibodies against HBsAg or HBV core antigen. Seven patients who were negative for those antibodies were also negative for HBV DNA by the PCR. These results suggest that HBV may be involved in the etiology of the liver disease of some patients with what is presently classified as non-A, non-B hepatitis, if they are positive for HBV antibodies.
Similar content being viewed by others
References
Szmuness W: Hepatocellular carcinoma and the hepatitis B virus; evidence for a causal association. Prog Med Virol 1978;24:40–69.
Beasley RP: Hepatitis B virus as the etiologic agent in hepatocellular carcinoma: epidemiologic considerations. Hepatology 1982;2:21S-26S.
Sakamoto M, Hirohashi S, Tsuda H, et al: Increasing incidence of hepatocellular carcinoma possibly associated with non-A, non-B hepatitis in Japan, disclosed by hepatitis B virus DNA analysis of surgically resected cases. Cancer Res 1988;48:7294–7297.
Goudeau A, Maupas Ph, Dubois F, et al: Hepatitis B infection in alcoholic liver disease and hepatocellular carcinoma in France. Prog Med Virol 1981;27:26–34.
Okuda H, Obata H, Motoike T, et al: Clinicopafhological features of hepatocellular carcinoma-Comparison of hepatitis B seropositive and seronegative patients. Hepato-gastroenterol 1984;31:64–68.
Iwama S, Ohnishi K, Nakajima Y, et al: A clinical study of hepatocellular carcinoma (HCC) in relation to hepatitis B seromarkers. Implications of non-A, non-B hepatitis (NANB). Hepatology 198;2:117.
Hoofnagle JH, Seeff LB, Bales ZB, et al: Type B hepatitis after transfusion with blood containing antibody to hepatitis B core antigen. N Engl J Med 1978;298:1379–1383.
Saiki RK, Scharf S, Faloona F, et al: Enzymatic amplification of ßglobin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985;230:1350–1354.
Kogan SC, Doherty M, Gitschier J: An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences: N Engl J Med 1987;317:985–990.
Saiki RK, Gelfand DH, Stoffel S, et al: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487–491.
Kinoshita T, Shimoyama M, Tobinai K, et al: Detection of mRNA for the taxi /rex 1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult-T-cell leukemia patients and viral carriers by using the polymerase chain reaction. Proc Natl Acad Sci USA 1989;86:5620–5624.
Lee H, Swanson P, Shorty VS, et al: High rate of HTLV-II infection in seropositive IV drug abusers in New Orleans. Science 1989;244: 471–475.
Greenberg SJ, Ehllich GD, Abbot MA, et al: Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification. Proc Natl Acad Sci USA 1989;86:2878–2882.
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975;98:503–517.
Fujiyama A, Miyanohara A, Nozaki C, et al: Cloning and structural analysis of hepatitis B virus DNAs, subtype adr. Nucleic Acid Res 1983;11:4601–4610.
Brèchot C, Degos F, Lugassy C, et al: Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen. N Engl J Med 1985;312:270–276.
Figus A, Blum HE, Vyas GN, et al: Hepatitis B viral nucleotide sequences in non-A, non-B or hepatitis B virus related chronic liver disease. Hepatology 1984;5:364–368.
Hino O, Kitagawa T, Koike K, et al. Detection of hepatitis B virus DNA in Hepatocellular carcinoma in Japan. Hepatology 1984;4:90–95.
Brèchot C, Nalpas B, Courouce AM, et al: Evidence that hepatitis B virus has a role in liver cell carcinoma in alcoholic liver disease. N Engl J Med 1982;306:1384–1387.
Kaneko S, Miller RH, Feinstone SM, et al: Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proc Natl Acad Sci USA 1989;86: 312–316.
Nagaya T, Nakamura T, Tokino T, et al: The mode of hepatitis B virus DNA integration in chromosomes of human hepatocellular carcisnoma. Genes Dev 1987; 1:773–782.
Yaginuma K, Kobayashi H, Kobayashi M, et al: Multiple integration site of hepatitis B virus DNA in hepatocellular carcinoma and chronic active hepatitis tissues from children. J Virol 1987;61:1808–1813.
Choo QL, Kuo G, Weiner AJ, et al: Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989;244:359–362.
Kuo G, Choo QL, Alter HJ, et al: An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 1989;244:362–364.
Miyamura T, Saito I, Katayama T, et al: Detection of antibody against antigen expressed by molecularly cloned Hepatitis C virus cDNA: Application to diagnosis and blood screening for posttransfusion hepatitis. Proc Natl Acad Sci USA 1990;87:983–987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohkoshi, S. Detection of HBV DNA in non-A, non-B hepatic tissues using the polymerase chain reaction assay. Gastroenterol Jpn 26, 728–733 (1991). https://doi.org/10.1007/BF02782860
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02782860