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A microspectrophotometric study of DNA ploidy patterns of colorectal adenomas

Gastroenterologia Japonica volume 26, pages 721–727 (1991)Cite this article

Summary

Eighty-four adenomas (31 mild, 25 moderate, 28 severe atypia), 32 invasive carcinomas and 15 samples of normal mucosa were collected form polypectomy and surgically resected specimens, and their DNA ploidy patterns were examined by microspectrophotometry. The frequency of polyploidy and aneuploidy were 7% and 0% in normal mucosa, 13% and 0% in mild atypia, 20% and 44% in moderate atypia, 32% and 22% in severe atypia and 31% and 53% in invasive carcinomas, respectively. Furthermore, these abnormal ploidy patterns were found frequently in moderate atypia accompanying severe atypia and mild atypia accompanying moderate atypia. These findings suggest that adenomas with moderate atypia might be the early stage of histologically overt cancer. The thin-section method (4 μm or 7μm) was useful for measuring regional DNA ploidy patterns of colorectal adenoma.

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References

  1. Rognum TO, Fausa O, Brandtzaeg P: Immunohistochemical evaluation of carcinoembryonic antigen, secretory component, and epithelial IgA in tubular and villous large-bowel adenomas with different grades of dysplasia. Scand J Gastroenterol 1982; 17:341–348.

    PubMed  CAS  Article  Google Scholar 

  2. O’brien MJ, Zamcheck N, Burke B, et al: Immunocytochemical localization of carcinoembryonic antigen in benign and malignant colorectal tissues. Assessment of diagnostic value. Am J Clin Pathol 1981;75:283–290.

    PubMed  CAS  Google Scholar 

  3. Yonezawa S, Nakamura T, Tanaka S, et al: Glycoconjugate with Ulex europaeus agglutinin-1-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel. JNC1 1982;69:777–785.

    CAS  Google Scholar 

  4. Boland CR, Montgomery CK, Kim YS: A cancer-associated mucin alteration in benign colonie polyps. Gastroenterology 1982;82:664–672.

    PubMed  CAS  Google Scholar 

  5. Abe K, Hakomori S, Ohshiba S: Differential expression of difucosyl type 2 chain (LeY) defined by monoclonal antibody AH6 in different locations of colonic epithelia, various histological types of colonie polyps, and adenocarcinoma. Cancer Res 1986;46:2639–2644.

    PubMed  CAS  Google Scholar 

  6. Ookura Y, Nakamura K: Histopathological study on the histogenesis of colorectal carcinoma. Stomach and Intestine 1987;22:431–441. (in Japanese with English abstract)

    Google Scholar 

  7. Hedley DW, Friedlander ML, Taylor IW, et al: Method for analysis of cellular DNA content in paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 1983;31:1333–1335.

    PubMed  CAS  Google Scholar 

  8. Masaki T, Muto T, Suzuki K, et al: A microspectrophotometric study of colorectal adenomas. Stomach and Intestine 1989;24:308–316. (in Japanese with English abstract)

    Google Scholar 

  9. Morson BC, Sobin LH: Histological typing of intestinal tumours: International Histological Classification of Tumours, No. 15. World Health Organization, Geneva. 1976.

    Google Scholar 

  10. Takamatsu T, Nakanishi K, Onouchi Z, et al: Nonspecific (“Pseudoplasmal”) dye binding in the Feulgen nuclear stain and its blocking by Azocarmin G. Histochemistry 1980;66:169–180.

    PubMed  Article  CAS  Google Scholar 

  11. Willbanks GD, Richart RM, Terner JY: DNA content of cervical intraepithelial neoplasia studied by two-wavelength Feulgen cytophotometry. Am J Obst & Gynec 1967;98:792–799.

    Google Scholar 

  12. Silviano Brandao HJ: DNA content in epithelial cells of dysplasias of the uterine cervix. Histologic and microspectrophotometric observations. Acta Cytologica 1969;13:232–237.

    Google Scholar 

  13. Hrushovetz SB, Lauchlan SC: Comparative DNA content of cells in the intermediate and parabasal layers of cervical intraepithelial neoplasia studied by two-wavelength Feulgen cytophotometry. Acta Cytologica 1970; 14:68–77.

    PubMed  CAS  Google Scholar 

  14. Wagner D, Sprenger E, Blank MH: DNA-content of dysplastic cells of the uterine cervix. Acta Cytologica 1972; 16:517–522.

    PubMed  CAS  Google Scholar 

  15. Nasiell M, Kato H, Auer G, et al: Cytomorphological grading and Feulgen DNA-analysis of metaplastic and neoplastic bronchial cells. Cancer 1978;41:1511–1521.

    PubMed  Article  CAS  Google Scholar 

  16. Mukada T, Sasano N, Sato E: Evaluation of esophageal dysplasia by cytofluorometric analysis. Cancer 1978;41:1399–1404.

    PubMed  Article  CAS  Google Scholar 

  17. Koga Y, Sugimachi K, Kuwano H, et al: Cytophotometric DNA analysis of esophageal dysplasia and carcinoma induced in rats by NMethyl-N-amylnitrosamine. Eur J Cancer Clin Oncol 1988;24:643–651.

    PubMed  Article  CAS  Google Scholar 

  18. Weiss H, Wildner GP, Jacobasch KH, et al: Characterization of human adenomatous polyps of the colorectal bowel by means of DNA distribution patterns. Oncology 1985;42:33–41.

    PubMed  CAS  Article  Google Scholar 

  19. Van den Lngh HF, Griffioen G, Cornellisse CJ: Flow cytometric detection of aneuploidy in colorectal adenomas. Cancer Res 1985;45: 3392–3397.

    Google Scholar 

  20. Goh HS, Jass JR: DNA content and adenoma-carcinoma sequence in the colorectum. J Clin Pathol 1986:39;387–392.

    PubMed  Article  CAS  Google Scholar 

  21. Quirke P, Fozard JBJ, Dixon MF, et al: DNA aneuploidy in colorectal adenomas. Br J Cancer 1986;53:477–481.

    PubMed  CAS  Google Scholar 

  22. Banner BF, Chacho MS, Roseman DL, et al: Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol 1987;87:313–318.

    PubMed  CAS  Google Scholar 

  23. Giaretti W, Sciallero S, Bruno S, et al: DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas. Cytometry 1988;9:238–244.

    PubMed  Article  CAS  Google Scholar 

  24. Tsubone M: DNA content of large intestinal adenoma. Gann 1982;73: 413–419.

    PubMed  CAS  Google Scholar 

  25. Jarvis LR, Graff PS, Whitehead R: Correlation of nuclear ploidy with histology in adenomatous polyps of colon. J Clin Pathol 1987;40:26–33.

    PubMed  Article  CAS  Google Scholar 

  26. Matthews J, Sousha S, Parkins RA, et al: Proliferation patterns and aneuploidy in adenomatous polyps of the colon. Br J Surg 1988;75:906–909.

    PubMed  Article  CAS  Google Scholar 

  27. Whitehead F, Jarvis LR, Skinner JM, et al: Ploidy studies in adenomatous polyps of the colon. J Clin Pathol 1985;38:1261–1264.

    PubMed  Article  CAS  Google Scholar 

  28. Hamada S, Namura K, Itoh R, et al: Characteristics of colorectal epithelia and adenomas as revealed by DNA cytofluorometry. Jpn J Cancer Res (Gann) 1987;78:826–832.

    CAS  Google Scholar 

  29. Muto T: Serial section study of colonie adenomas with special reference to minute carcinoma. Jpn J Cancer Res 1989;80;356–359.

    PubMed  CAS  Google Scholar 

  30. Sawada T, Muto T, Morioka Y: Borderline lesion of the colon; A study of electron microscopic features of dysplasia in colonie adenomas. Progress of Digestive Endoscopy 1981;19:28–34. (in Japanese with English abstract)

    Google Scholar 

  31. Bos JL, Fearon ER, Hamilton SR, et al: Prevalence of ras gene mutations in human colorectal cancers. Nature 1987;327:293–297.

    PubMed  Article  CAS  Google Scholar 

  32. Swartz FJ: The development in the human liver of multiple Desoxyribose Nucleic Acid (DNA) classes and their relationship to the age of the individual. Chromosoma 1956;Bd. 8,S:53–72. The authors are grateful to Dr. Toshio Sawada for helpful discussions and Mrs. Masako Kuwahara for technical assistance.

    PubMed  Article  CAS  Google Scholar 

  33. Atkin NB, Ross AJ: Polyploidy in human tumours. Nature 1960;187: 579–581.

    PubMed  Article  CAS  Google Scholar 

  34. Kiefer G, Sandritter W: DNA and the cell cycle. Beitr Path 1976;Bd. 158;332–362.

    CAS  Google Scholar 

  35. Weiss H, Wildner GP, Gutz HJ, et al: DNA distribution patterns of preneoplastic cells and their interpretation. Oncology 1981;38:210–218.

    PubMed  CAS  Google Scholar 

  36. Carpenter R, Cooke T, Matthews J, et al: Does flow cytometry accurately determine tumour ploidy? Br J Surg 1988;75:1263.

    Google Scholar 

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Affiliations

  1. First Department of Surgery, University of Tokyo Faculty of Medicine, 7-3-1 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Tadahiko Masaki M.D., Tetsuichiro Muto, Kimitaka Suzuki & Yasuhiko Morioka

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  1. Tadahiko Masaki M.D.
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  2. Tetsuichiro Muto
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  3. Kimitaka Suzuki
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  4. Yasuhiko Morioka
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Masaki, T., Muto, T., Suzuki, K. et al. A microspectrophotometric study of DNA ploidy patterns of colorectal adenomas. Gastroenterol Jpn 26, 721–727 (1991). https://doi.org/10.1007/BF02782859

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  • DOI: https://doi.org/10.1007/BF02782859

Key words

  • colorectal adenoma
  • DNA ploidy pattern
  • microspectrophotomet