Skip to main content
SpringerLink
Log in

Impaired pancreatic exocrine function in rats with carbon tetrachloride-induced liver cirrhosis

  • Original Articles
  • Published:
International Journal of Gastrointestinal Cancer Aims and scope Submit manuscript

Summary

Background

Substantial numbers of studies have revealed the close correlation between chronic pancreatitis and cirrhosis in human. However, the situation with regard to pancreatic enzyme secretion is less clear.

Aim

The aim of the study was to investigate pancreatic exocrine function in rat with carbon tetrachloride-induced liver cirrhosis in rats.

Methods

Pancreatic exocrine function and morphology in Sprague-Dawley rats with carbon tetrachloride-induced liver cirrhosis were investigated. Pancreatic exocrine functions stimulated by cholecystokinin-8 and other secretagogs were assessed in isolated pancratic acini, and in vivo and morphological changes were studied by routine were assessed in isolated pancreatic acini, and in vivo and morphological changes were studied by routine histological examination and electron microscopy.

Results

The basal and cholecystokinin-8-stimulated amylase releases from acini and acinar amylase content were significantly lower in the cirrhotic rats than the control. None of the secretagogs induced the some amount of amylase release in cirrhotic as in control rats. Volume of the pancreatic juice and outputs of amylase and protein were significantly decreased under basal and cholecystokinin-8-stimulated conditions in vivo. Electron microscopy revealed most of the rough-surfaced endoplasmic reticulum accompanying less numbers of ribosomes to be dilated and some mitochondria to be swollen in cirrhotic rats.

Conclusion

Pancreatic exocrine functions are decreased in cirrhotic rats owing to alterations at the electron microscopic levels, reflecting an impaired acinar intracellular messenger system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brailski C, Mendisova A, Glawintschewa I. Pancreatic exocrine secretion in patients with cirrhosis of the liver.Dtsch Z Verdau Stoffwechselkr 1981; 41: 213–219.

    PubMed  CAS  Google Scholar 

  2. Caradonna P, Costamagna G, Benedetti G, Gentiloni N, Gasbarrini GB. Chronic pancreatitis prevalence in liver cirrhosis.Ital J Gastroenterol 1996; 28: 91–94.

    PubMed  CAS  Google Scholar 

  3. Gregg JA, Sharma MM. Pancreatic hypersecretion in liver disease.Am J Dig Dis 1978; 23: 9–11.

    Article  PubMed  CAS  Google Scholar 

  4. Greiner L, Shubert E, Franken FH. Koinzidenz von chronischer Pankreatitis und Leberzirrhose bei Alkoholabusus. Eine rontgen und histologische Studie.Z Gastroenterol 1978; 80: 998–1003.

    Google Scholar 

  5. Hayakawa T, Kondo T, Shibata T, Kitagawa M, Sakai Y, Sobajima H, et al. Exocrine pancreatic function in chronic liver diseases.Am J Gastroenterol 1991; 86: 201–204.

    PubMed  CAS  Google Scholar 

  6. Stinson JC, Bagenstoss AH, Morlock CG. Pancreatic lesions associated with cirrhosis of the liver.Am J Clin Pathol 1952; 22: 117–123.

    PubMed  Google Scholar 

  7. Lee SP, Lai KS. Exocrine pancreatic function in hepatic cirrhosis.Am J Gastroenterol 1976; 65: 244–248.

    PubMed  CAS  Google Scholar 

  8. Olmo Martinez ML, Bermejo MB. Changes in pancreatic secretion in alcoholic liver disease.Rev Esp Enferm Dig 1990; 77: 197–204.

    PubMed  Google Scholar 

  9. Renner IG, Rinderknecht H, Wisner JR. Pancreatic secretion after secretin and cholecystokinin stimulation in chronic alcoholics with and without cirrhosis.Dig Dis Sci 1983; 28: 1089–1093.

    Article  PubMed  CAS  Google Scholar 

  10. Cummins AJ, Bockus HJ. Abnormal serum pancreatic enzyme values in liver disease.Gastroenterology 1951; 18: 518–527.

    PubMed  CAS  Google Scholar 

  11. Kataev SS, Vasil'eva NS, Avdeev VG, Gil'var LL, Starodvortseva VG, Zairat'iants OV. Exocrine function of the pancreas in patients with chronic hepatitis and liver cirhosis of various etiologies.Klin Med 1993; 71: 37–42.

    CAS  Google Scholar 

  12. Sobel HJ, Waye JH. Pancreatic changes in various types of cirrhosis in alcoholics.Gastroenterology 1963; 45: 341–344.

    PubMed  CAS  Google Scholar 

  13. Munoz TE, Bouza JJP, Bravo AL, Hernandez MMA, Marino EC. Experimental thioacetamide-induced cirrhosis of the liver.Histol Histopathol 1991; 6: 95–100.

    Google Scholar 

  14. Pap A, Varro A. Pancreatic amylase— and protein—concentrations associated with thioacetamide-induced cirrhosis of the liver in rats.Acta Med Acad Sci Hung 1978; 35: 311–316.

    PubMed  CAS  Google Scholar 

  15. Santamaria G, de Andres L, Fernandez A, Gonzalez FE, Blanco VFJ, Coca G, Gome AC. Modifications of the enzymatic content and morphology of the exocrine pancreas of rats with experimental hepatic cirrhosis induced by thioacetamide.Ann Med Intern 1991;8: 601–604.

    Google Scholar 

  16. Fischer-Nielsen A., Poulsen HE, Hansen BA, Hage E, Keiding S. CC14 cirrhosis in rats: irreversible histological changes and differentiated functional impairment.J Hepatol 1991; 12: 110–117.

    Article  PubMed  CAS  Google Scholar 

  17. Nakamura T, Otsuki M, Tani S, Okabayashi Y, Fujii M, Oka T, et al. Pancreatic endocrine function in cirrhotic rats.Metabolism 1988; 37: 892–899.

    Article  PubMed  CAS  Google Scholar 

  18. Wu J, Lindstrom P, Danielsson A, Sehlin J. Insulin secretion in pancreatic islets from rats with cirrhosis.J Hepatol 1994; 21: 332–339.

    Article  PubMed  CAS  Google Scholar 

  19. Williams JA, Yule DI. Stimulus—secretion coupling in pancreatic acinar cells, inThe Pancreas, Biology, Pathobiology, and Diseae. Go VLW, Lebenthal E, DiMagno EP, Reber HA, Gardner JD, Scheele GA, eds. Raven, New York, 1993, pp. 167–189.

    Google Scholar 

  20. Doi K, Kurabe S, Shimazu N, Inagaki M. Systemic histopathology of rats with CC14-induced hepatic cirrhosis.Lab Anim 1991; 25: 21–25.

    Article  PubMed  CAS  Google Scholar 

  21. Williams JA, Korc M, Dormer RL. Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini.Am J Physiol 1978; 235: 517–524.

    PubMed  CAS  Google Scholar 

  22. Ceska M, Birath K, Brown B. A new and rapid method for the clinical determination of alpha-amylase activities in human serum and urine. Optimal conditions.Clin Chim Acta 1969; 26: 437–444.

    Article  PubMed  CAS  Google Scholar 

  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent.J Biol Chem 1951; 193: 265–275.

    PubMed  CAS  Google Scholar 

  24. Inagaki T, Hoshino M, Ohara H, Yamada T, Ogasawara T, Shimizu H, et al. Decreased pancreatic exocrine function in the Mutant Eisai Hyperbilirubinemic rat (EHBR).Pancreas 1999; 18: 172–179.

    Article  PubMed  CAS  Google Scholar 

  25. Tateishi K, Hamaoka T, Sugiura N, Yanaihara C, Yanaihara N. A novel immunization procedure for production of anti-cholecystokinin-specific antiserum of low cross-reactivity.J Immunol Methods 1981; 47: 249–258.

    Article  PubMed  CAS  Google Scholar 

  26. Bruzzone R. The molecular basis of enzyme secretion.Gastroenterology 1990; 99: 1157–1176.

    PubMed  CAS  Google Scholar 

  27. Pandol SJ, Schoeffield MS, Sachs G, Muallem S. Role of free cytosolic calcium in secretagogue-stimulated amylase release from dispersed acini from guinea pig pancreas.J Biol Chem 1985; 260: 10,081–10,086.

    CAS  Google Scholar 

  28. de Pont JJHHM, Fleuren-Jakobs AMM. Synergistic effect of A23187 and a phorbol ester on amylase secretion from rabbit pancreatic acini.FEBS Lett 1984; 170: 64–68.

    Article  PubMed  Google Scholar 

  29. Burnham DB, Munowitz P, Hootman SR, Williams JA. Regulation of protein phosphorylation in pancreatic acini.Biochem J 1986; 235: 125–131.

    PubMed  CAS  Google Scholar 

  30. Wrenn RW. Phosphorylation of a pancreatic zymogen granule membrane protein by endogenous calcium/phospholipid-dependent protein kinase.Biochim Biophys Acta 1984; 775: 1–6.

    Article  PubMed  CAS  Google Scholar 

  31. Scheele GA, Kern HF. Cellular compartmentation, protein processing, and secretion in the exocrine pancreas, inThe Pancreas, Biology, Pathobiology, and Disease. Go VLW, Lebenthal E, DiMagno EP, Reber HA, Gardner JD, Scheele GA, eds. Raven, New York, 1993, pp. 121–150.

    Google Scholar 

  32. Watari N. Symposium on the alterations of cell organelles: Rough-surfaced endoplasmic reticulum.J Clin Electron Microscop 1979; 12: 463–464.

    Google Scholar 

  33. Furukawa T, Yasumoto K, Inokuchi K. Pulmonary interstitial edema in experimental cirrhosis of the liver in rats.Eur Surg Res 1984; 16: 366–371.

    Article  PubMed  CAS  Google Scholar 

  34. Miyasaka K, Funakoshi A, Shikado F, Kitani K. Stimulatory and inhibitory effects of bile salts on rat pancreatic secretion.Gastroenterology 1992; 102: 598–604.

    PubMed  CAS  Google Scholar 

  35. Nagy I, Hajnal F, Mohacsi G, Nemeth J, Laszik Z, Pap A. Pancreatic trophism in experimental liver cirrhosis.Int J Pancreatol 1993; 14: 157–166.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Internal Medicine, Nagoya City University, Medical School, 1 Kawasumi, Mizuhocho, Mizuho-ku, 467-0801, Nagoya, Japan

    Tetsuya Ogasawara, Takanori Inagaki, Tamaki Yamada, Hirotaka Ohara, Takahiro Nakazawa & Makoto Itoh

Authors
  1. Tetsuya Ogasawara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takanori Inagaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tamaki Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hirotaka Ohara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takahiro Nakazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Makoto Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ogasawara, T., Inagaki, T., Yamada, T. et al. Impaired pancreatic exocrine function in rats with carbon tetrachloride-induced liver cirrhosis. Int J Gastrointest Canc 26, 59–67 (1999). https://doi.org/10.1007/BF02781732

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02781732

Key Words

Search

Navigation