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Delayed luminescence of luminol initiated by a membrane-bound peroxidase

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Abstract

The luminescense of the luminol-H2O2 system was initiated by either free or membrane-bound horseradish peroxideae (HRP). The instantaneous luminescene decayed rapidly and was followed by the delayed luminescence in the presence of excess luminol. The delayed luminescence was characterized by a chain reaction, in which luminescence intensity increased exponentially. Membrane-bound HRP demonstrated that the delayed luminescence took place even in the absence of HRP if the instantaneous luminescence was initiated by HRP. A mechanism for the nonenzymatic luminescence is proposed and discussed.

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Authors and Affiliations

  1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Midori-ku, 227, Yokohama, Japan

    Yoshihito Ikariyama & Shuichi Suzuki

  2. Institute of Materials Science, University of Tsukuba, Sakura-mura, 305, Ibaraki, Japan

    Masuo Aizawa

Authors
  1. Yoshihito Ikariyama
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  2. Shuichi Suzuki
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  3. Masuo Aizawa
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Ikariyama, Y., Suzuki, S. & Aizawa, M. Delayed luminescence of luminol initiated by a membrane-bound peroxidase. Appl Biochem Biotechnol 6, 223–235 (1981). https://doi.org/10.1007/BF02780800

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  • DOI: https://doi.org/10.1007/BF02780800

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