Summary
The relative abundance of rat aldolase A, B, C, α-fetoprotein, and albumin mRNAs was determined by Northern hybridization during liver regeneration after partial hepatectomy. Aldolase A mRNA increased more than 10-fold on the 3rd day after partial resection compared with that of normal adult rat liver. S1 analysis revealed that three species of aldolase A mRNAs (mRNA I, II & III) reappeared. However, transcriptional rate of aldolase A mRNA did not change at all during the regeneration. In contrast, aldolase B, aldolase C and albumin mRNAs did not change at all. These findings suggest that the differentiated hepatocyte maintains a differentiated state during the liver regeneration as seen in aldolase B, whereas “oncofetal” isozymes such as aldolase A resurge after partial hepatectomy under the control of posttranscriptionla mechanism.
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Abbreviations
- AFP:
-
a-fetoprotein
- ALB:
-
albumin
- cDNA:
-
complementary DNA
- lxDenhardt:
-
0.02% bovine serum albumin
- 0.02%:
-
ficoll
- 0.02%:
-
polyvinyl-pyrolidone
- Kb:
-
kilobase
- oligo (dT) cellulose:
-
oligo deoxythymidylate cellulose
- poly (A)+RNA:
-
polyadenylated RNA
- SDS:
-
sodium dodecyl sulfate
- SSC:
-
standard saline citrate
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This investigation was supported in part by a grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
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Motomura, M., Mukai1, T., Ozaki, I. et al. Transcriptional regulation of an aldolase gene in the regenerating rat liver. Gastroenterol Jpn 25, 350–355 (1990). https://doi.org/10.1007/BF02779450
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DOI: https://doi.org/10.1007/BF02779450