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Gastroenterologia Japonica

, Volume 23, Issue 4, pp 408–413 | Cite as

Interleukin 2 receptor bearing T lymphocytes in patients with chronic liver disease

  • Shinichi Kakumu
  • Kentaro Yoshioka
  • Akihiko Fuji
  • Hirofumi Tahara
Original Article
  • 12 Downloads

Summary

We investigated the role of interleukin 2 receptor expression (IL 2R) on T cell in chronic liver disease. IL 2R was determined by analysing T cell surface Tac antigen with anti-Tac, a monoclonal antibody that binds at or near the binding site for IL 2, using a fluorescence-activated cell sorter. The percentage of Tac+ cells in T cell fraction from peripheral blood mononuclear cells in unstimulated cultures was 7.9 ±2.1% (±SD) in controls. A similar value was obtained in asymptomatic carriers of HBsAg (ASC), in patients with chronic active hepatitis (CAH) and liver cirrhosis (LC). Upon stimulatin with recombinant IL 2, there was a small but significant increase in Tac+ cells. The percentages of Tac+ cells in IL 2-stimulated cultures were significantly lower in ASC (P<0.01), patients with CAH (P<0.01) and LC (P<0.05) as compared to controls (16.4 ±4.4%). Percentages of Tac+ cells after stimulation with phytohemagglutinin P (PHA-P) in ASC and CAH did not differ from controls (74.9 ±5.9%). Only patients with LC showed diminished Tac+ cells (P<0.01) compared to controls. During a 4-wk course of recombinant IL 2 therapy, a serial study on 5 HBeAg-positive patients with CAH was done, and the results showed that Tac+ cells were significantly diminished 2 wk (P<0.01) and 4 wk (P<0.05) after starting therapy in comparison with pretreatment levels in IL 2-stimulated cultures. OKT3+, OKT4+, OKT8+ cells in T cell culture in response to stimulation with PHA-P were significantly decreased in patients with CAH and LC when compared with controls. These results indicate that IL 2/ IL 2R system has a role on modulating immune resoponse in chronic liver disease.

Key words

Chronic liver disease Interleukin 2 receptor T lymphocyte 

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Copyright information

© The Japanese Society of Gastroneterology 1988

Authors and Affiliations

  • Shinichi Kakumu
    • 1
  • Kentaro Yoshioka
    • 1
  • Akihiko Fuji
    • 1
  • Hirofumi Tahara
    • 1
  1. 1.Third Department of MedicineNagoya University School of MedicineNagoyaJapan

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