Summary
Lymphoid cell subsets, including T cells as well as Ig-containing cells in the colonic mucosa and HLA-DR antigens on colonie epithelia, were examined in non-IBD colitis (colitis excluding ulcerative colitis (UC) and Crohn’s disease) by the indirect immunoperoxidase staining method. Mouse anti-CD5, CD8, CD4, IgG, IgAl, IgA2, IgM, IgD, IgE, HLA-DR, and Nula monoclonal antibodies were used as the first antibody. The results were compared to those of the normal controls and UC. T cell subsets in non-IBD colitis were almost similar to those of the controls and UC. The number of Ig-containing cells of all classes, except for IgA, tended to be increased in non-IBD colitis. In particular, both IgG- and IgE-containing cells were significantly increased compared to those in the controls. Compared to UC, IgG-containing cells were decreased in non-IBD colitis. Namely, in non-IBD colitis, as well as in UC, the change of Ig-containing cells (B cell lineage) was more pronounced than that of T cells. The frequency of the expression of HLA-DR antigens on colonie epithelia in non-IBD colitis was 70%, which was significantly higher than that in controls (0%), but significantly lower than that in UC (100%). Whether the differences in the number of IgG-containing cells, and the frequency of epithelial HLA-DR expression between non-IBD colitis and UC was due to the differences of the degree of local inflammation or due to the differences of the nature of the two diseases was not elucidated in this study.
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References
Brandtzaeg P, Baklien K, Fausa O, et al: Immunohistochemical characterization of local immunoglobulin formation in ulcerative colitis. Gastroenterology 1974;66:1123–1136
Rosekrans PCM, Meijer CJL, Vanderwal AM, et al: Immunoglobulin containing cells in inflammatory bowel disease of the colon: a morphometric and immunohistochemical study. Gut 1980;21:941–947
Lloid G, Green FHY, Fox H, et al: Mast cells and immunoglobulin E in inflammatory bowel disease. Gut 1975;16:861–866
Selby WS, Janossy G, Bofill M, et al: Intestinal lymphocyte subpopulations in inflammatory bowel disease: an analysis by immunohistological and cell isolation techniques. Gut 1984;25: 32–40
Ohta H: Studies of lymphoid cell subsets of colonie mucosa in ulcerative colitis. Jpn J Gastroenterol 1986:83:1143–1152 (in Japanese)
Miyazaki H, Kawasaki H, Hirayama H: Studies on lymphocyte subpopulations in human colonic biopsy specimens by colonoscopy. Dig Dis Sei 1985:30:143–148
Scott BB, Goodall A, Stephenson P, et al: Rectal mucosal plasma cells in inflammatory bowel disease. Gut 1983:24:519–524
Van Spreeuwel JP, Lindeman J, Meijer C: A quantitative study of immunoglobulin containing cells in the differential diagnosis of acute colitis. J Clin Pathol 1985:38:774–777
Miyahara T: Serum immunoglobulin level and local immune response of ischemic colitis and antibiotic associated colitis. Jpn J Gastroenterol 1987;84:661–669 (in Japanese)
Londei M, Bottazzo GF, Fcldomann M: Human T-cell clones from autoimmune thyroid glands: specific recognition of autologous thyroid cells. Science 1985:228:85–89
Foulis AK: Class II major histocompatibility complex and organ specific autoimmunity in man. J Pathol 1986;150:5–11
Selby WS, Janossy G, Mason DY, et al: Expression of HLA-DR antigens by colonie epithelium in inflammatory bowel disease. Clin Exp Immunol 1983:53:614–618
Iizuka M, Chiba M, Ohta H, et al: Expression of HLA-DR antigens on colonic epithelium in ulcerative colitis. Gastroenterol Jpn 1987:22:571–577
Daar AS, Fuggle SV, Ting A, et al: Anomolous expression of HLA-DR antigens on human colorectal cancer cells. J Immunol 1982:129, 447–449
McDonald GB, Jewell DP: Class II antigen (HLA-DR) expression by intestinal epithelial cells in inflammatory diseases of colon. J Clin Pathol 1987:40:312–317
Iizuka M, Chiba M, Ohta H, et al: Lymphoid cell subsets in colonic mucosa in colitis excluding ulcerative colitis and Crohn’s disease. Digestive Organ & Immunology 1987; 19:36–39 (in Japanese)
Chiba M: Histocompatibility antigen and gastrointestinal diseases. — HLA-DR antigens on intestinal epithelia — Digestive Organ & Immunology 1989:22:255–261 (in Japanese)
Isobe Y, Chen ST, Nakane PK, et al: Studies on translocation of immunoglobulins across intestinal epithelium 1. Improvements in the peroxidase-labeled antibody methods for application to study of human intestinal mucosa. Acta Histochem Cytochem 1977:10:161–171
Ledbetter JA, Evans RL, Lipinski M, et al: Evolutionary conservation of surface molecules that distinguish T lymphocyte hclper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med 1981:153:310–323
Jefferis R, Reimer CB, Skvaril F, et al: Evaluation of monoclonal antibodies having specificity for human IgG subclasses: Results of an IUIS/WHO collaborative study. Immuno Lett 1985:10:223–252
Conley ME, Kearney JF, Lawton AR. et al: Differentiation of human B cells expressing the IgA subclasses as demonstrated by monoclonal hybridioma antibodies. J Immunol 1980:125: 2311–2316
Kuritani T, Cooper MD: Human B cell differentiation I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J Exp Med 1982:155:839–851
Lampson LA, Levy R: Two populations of la-like molecules on human B cell line. J Immunol 1980:125:293–299
Matsumoto K: Studies of monoclonal antibodies specific to human leucocyte differentiation antigens. J Kurume Med Assoc 1984:47:1382–1397 (in Japanese)
Chiba M, Ohta H, Nagasaki A, et al: Lymphoid cell subsets in normal human small intestine. Gastroenterol Jpn 1986;21: 336–343
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This work was partly supported by a grant from the Research Committee for Intractable Intestinal Disease, Ministry of Health and Welfare, Japan.
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Iizuka, M., Chiba, M., Horie, Y. et al. Lymphoid cell subsets in colonie mucosa and HLA-DR antigens on colonic epithelia in colitis excluding ulcerative colitis and crohn’s disease. Gastroenterol Jpn 25, 700–707 (1990). https://doi.org/10.1007/BF02779183
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DOI: https://doi.org/10.1007/BF02779183