Summary
The present study was conducted to determine whether the leucocyte adherence inhibition (LAI) assay was dependent on cell-mediated immunity and whether it was of potential use in diagnosis of pancreatic cancer. The LAI response was significantly positive in 15 PPD skin-test positive individuals but it was abated by pretreatment of sensitized mononuclear cells with anti-T cell antibody (OKT3) or by depletion of monocytes. Therefore, the LAI assay is probably related to cell-mediated immunity. The LAI assay was positive in 13 of 16 patients with pancreatic cancer using pancreatic cancer extract; however, a positive reaction was not found in 11 cases of chronic pancreatitis and 10 cases of hepatocellular carcinoma. Thus, the LAI assay appears rather specific. Mononuclear cells of pancreatic and gastric cancer patients showed LAI reactivity to autologous tumor extracts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Halliday WJ, Millers: Leucocyte adherence inhibition: A simple test for cell-mediated tumor immunity and serum blocking factor. Int J Cancer 9: 477, 1972
Tataryn DN, et al: Leucocyte adherence inhibition for detecting specific tumor immunity in early pancreatic cancer. Lancet 1: 1020, 1978
Kumagai N, et al: Immunodiagnosis of pancreatic carcinoma and hepatoma by tube leucocyte adherence inhibition assay. Digestive Organ and Immunology 3: 205, 1979 (Jpn)
Matsumoto M: Leucocyte adherence inhibition test. in “Method in Immunological Experiments” No. 8, by the Japanese Society for Immunology, Kanazawa, 1975, p 2507 (Jpn)
Lampert F, et al: A leucocyte adherence inhibition test for cell-mediated immunity. Klin Wschr 51: 198, 1973
Fujisawa T, et al: Leucocyte adherence inhibition by soluble tumor antigens in breast cancer patients. Cancer 39: 506, 1977
Yoshida T: Mechanism of leucocyte adherence inhibition, in “Regulatory Mechanism in Lymphocyte Activation” by David OL, New York, 1977, p 665
Holt PG, et al: The LAI microtest: A rapid and sensitive procedure for the demonstration of cell-mediated immunity in vitro. J Immunol Methods 8: 277, 1975
Creemers P: The role of leucocyte subpopulations in the indirect leucocyte adherence inhibition assay in the mammary tumor virus system. Eur J Immunol 7: 48, 1977
Yu DTY, et al: Peripheral blood Ia-positive T cells: Increase in certain diseases and after immunization. J Exp Med 15: 91, 1980
Ko HS, et al: Ia determinants on stimulated human T cells. J Exp Med 150: 246, 1979
Evans RL, et al: Peripheral human T cells sensitized in mixed leucocyte culture synthesize and express Ia-like antigens. J Exp Med 148: 1440, 1978
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Onji, M. Leucocyte adherence inhibition assay for cell-mediated immunity and immunodiagnosis of pancreatic cancer. Gastroenterol Jpn 19, 328–335 (1984). https://doi.org/10.1007/BF02779122
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02779122