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Serotonin directly stimulates luteinizing hormone-releasing hormone release from GT1-1 cells via 5-HT7 receptors

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Abstract

Luteinizing hormone-releasing hormone (LHRH release, which serves as the primary drive to the hypothalamic-pituitary gonaldal axis, is controlled by many neuro-mediators. Serotonin has been implicated in this regulation. However, it is unclear whether the central effect of serotonin on LHRH secretion is exerted directly on LHRH neurosecretory neurons or indirectly via multisynaptic pathways.

The present studies were undertaken in order to examine whether LHRH secretion from immortalized LHRH cell lines is directly regulated by serotonin and, if so, to identify the receptor subtype involved. 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), a 5-HT1A/7 receptor agonist, stimulated LHRH release from GT1-1 cells. This effect was blocked by ritanserin, a 5-HT2/7 receptor antagonist, but not by SDZ-216-525, a 5-HT1A antagonist. Basal LHRH release was not affected by the 5-HT2 agonist DOI.

Reverse transcription and polymerase chain reaction technique (RT-PCR) was used in order to identify 5-HT1A and 5-HT7 receptor mRNA in immortalized LHRH cell lines. GT1-1 cells express mRNA for the 5-HT7, but not the 5-HT1A receptor subtypes. These results demonstrate a direct stimulatory effect of serotonin on LHRH release via 5-HT7 receptor.

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Authors and Affiliations

  1. Laboratoire de Neuroendocrinologie Expérimentale. INSERM U297, Faculté de Médecine Nord, Bd Pierre Dramard, Institut Fédératif Jean Roche, 13916, Marseille Cedex 20, France

    Micheline Héry, Anne Marie François-Bellan, Francis Héry, Paule Deprez & Denis Becquet

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  1. Micheline Héry
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  2. Anne Marie François-Bellan
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  3. Francis Héry
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  4. Paule Deprez
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  5. Denis Becquet
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Correspondence to Micheline Héry.

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Héry, M., François-Bellan, A.M., Héry, F. et al. Serotonin directly stimulates luteinizing hormone-releasing hormone release from GT1-1 cells via 5-HT7 receptors. Endocr 7, 261–265 (1997). https://doi.org/10.1007/BF02778149

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  • DOI: https://doi.org/10.1007/BF02778149

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