Abstract
Insulin directly affects many aspects of cellular metabolism. An additional, poorly studied effect of insulin is the regulation of multiple genes whose products are important in many cellular functions. Using differential screening techniques, we identified insulin-regulated genes induced in insulin-treated rat H4IIE (H4) hepatoma cells. Two of the mRNAs identified were homologous to the previously characterized mousepip 92 and ratCL-6 immediate-early genes. Thepip 92 clone was initially isolated from mitogen-stimulated mouse Balb/c 3T3 fibroblasts, whereas theCL-6 clone was first isolated from regenerating rat liver. In this article, we demonstrated that in rat H4 cells, the transcription rates of bothpip 92 andCL-6 are induced by insulin alone. Additionally, we showed that the transcription rates of two other genes, whose sequences are not homologous to any other sequences in gene bank (“novel genes”), were rapidly and transiently induced by insulin. These results demonstrate that insulin regulates the expression of several novel genes with a time-course similar to members of the immediate-early response gene family.
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Bortoff, K.D., Zhu, CC., Hrywna, Y. et al. Insulin induction ofpip 92, CL-6, and novel mRNAs in rat hepatoma cells. Endocr 7, 199–207 (1997). https://doi.org/10.1007/BF02778142
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DOI: https://doi.org/10.1007/BF02778142