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Human pancreatic adenocarcinoma-associated antigens defined by novel murine monoclonal antibodies Pak-1 and Pak-2

Gastroenterologia Japonica volume 25, pages 236–243 (1990)Cite this article

Summary

Two murine monoclonal antibodies (MoAbs) Pak-1 and Pak-2 were established by immunizing Balb/c mice with human pancreatic adenocarcinoma xenografts, previously established in mice. Pak-1 showed a strong positive immunostaining to well- and moderately-differentiated tubular duct cell carcinoma of the pancreas but neither to poorly-differentiated nor to other non-tubular pancreatic carcinomas. Pak-2 showed a wide spectrum of immunostaining to ductal and islet cell carcinomas of the pancreas, revealing less reactivity to well-differentiated tubular duct cell carcinomas than Pak-1. The broad specificity of Pak-2 was similarly observed with extrapancreatic tumor tissues. Neither normal pancreatic tissues nor those with chronic pancreatitis were stained with Pak-1 and Pak-2, whereas the islet cells of normal pancreas were stained by both of them. Western blot analysis revealed that Pak-1 recognized two distinct glycoprotein molecules of ductal adenocarcinoma, 100K dalton molecular weight (MW) and pH6-7 isoelectric points (IP) on two dimensional electrophoresis, and that Pak-2 recognized three glycoprotein molecules, 35K dalton MW and pH7-10 IP. The treatment with periodic acid, neraminidase, trypsin and pronase revealed that antigenic epitopes of Pak-1 and Pak-2 may be composed of complex polysaccharide structure rather than terminal sialic acid residues.

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Affiliations

  1. The First Department of Surgery, Faculty of Medicine, Kagoshima University, 1208-1 Usuki-cho, 890, Kagoshima, Japan

    Kanro Makisumi M.D & Sonshin Takako

  2. Department of Virology, Kagoshima University Faculty of Medicine, 890, Kagoshima, Japan

    Kazuo Takahashi & Shunro Sonoda

Authors
  1. Kanro Makisumi M.D
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  2. Sonshin Takako
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  3. Kazuo Takahashi
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  4. Shunro Sonoda
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Additional information

This investigation was supported in part by Grants-in-Aid for Cancer Research (B. No. 60480307) from the Ministry of Education, Science and Culture of Japan.

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Makisumi, K., Takako, S., Takahashi, K. et al. Human pancreatic adenocarcinoma-associated antigens defined by novel murine monoclonal antibodies Pak-1 and Pak-2. Gastroenterol Jpn 25, 236–243 (1990). https://doi.org/10.1007/BF02776822

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  • DOI: https://doi.org/10.1007/BF02776822

Key words

  • glycoprotein antigen
  • murine monoclonal antibody
  • pancreatic adenocarcinoma