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Subgenomic studies on hepatitis B virus dna integrated into the genome of hepatocellular carcinoma

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Summary

Integration of hepatitis B virus (HBV) DNA into the chromosomal DNA of hepatocellular carcinoma (HCC) tissue was studied by the Southern blot analysis using probes for subgenomic regions C, PreS, S and X. Eighteen Hind III-fragments prepared from DNA of the tumor regions of six patients with HCC, and hybridized with a full genomic HBV probe were analyzed. Lack of region S or PreS was concurrently accompanied by deletion of their 3′-end region of the HBV minus strand DNA. Region C was missing in 11 out of 18 fragments while regions X, S and PreS were deleted in 5/18, 3/18 and 5/18 respectively. Therefore, in some sequences integrated by subgenomic HBV DNA, the integration of replicative intermediate of the HBV minus strand DNA into the chromosomal DNA may take place and one of the viral integration sites seemsto be in the region X.

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Authors and Affiliations

  1. First Department of Internal Medicine, Okayama University Medical School, Shikata-cho 2-5-1, 700, Okayama, Japan

    Kazumi Togawa M.D., Terukatsu Arima M.D. & Hideo Nagashima M.D.

Authors
  1. Kazumi Togawa M.D.
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  2. Terukatsu Arima M.D.
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  3. Hideo Nagashima M.D.
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Additional information

This study was partly reported at the 37th Annual Meeting of the American Association for the Study of Liver Diseases (Chicago, November 4, 1986).

This study supported by a grant-in-aid # 59480209 from the Ministry for education of Japan.

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Togawa, K., Arima, T. & Nagashima, H. Subgenomic studies on hepatitis B virus dna integrated into the genome of hepatocellular carcinoma. Gastroenterol Jpn 22, 621–626 (1987). https://doi.org/10.1007/BF02776723

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  • DOI: https://doi.org/10.1007/BF02776723

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