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Studies on the inhibition of experimental liver fibrosis

2. The mechanism of the inhibition of liver fibrosis of rats due to carbon tetrachloride by elastase

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Summary

In the previous experiments, it was demonstrated that high purity elastase extracted from porcine pancreas remarkably inhibits liver fibrosis of rats having chronic liver injury caused by carbon tetrachloride. This time, with the purpose to clarify the mechanism of inhibition of liver fibrosis by elastase, comparative study was made on the activity of lysosomal enzymes by measuring β-glucuronidase, cathepsin and collagenolytic activity, with the rats administered with elastase and with those untreated, during the period of development of liver fibrosis and the recovery from it.

In addition to it, in vitro experiments were made by having elastase act on the substrate comprising mixed collagen of acid soluble and neutral soluble collagens extracted from the skin of guinea pig and by observing collagen components by disc electrophoresis.

With any lysosomal enzymes, no marked difference was noticed between elastase group and non-administered group and thus the possibility of inhibition of liver fibrosis through activation of lysosomal enzyme by elastase was denied. The results of disc electrophoretic observation of the performance of elastase on collagen revealed that β-component of collagen is disappeared but α-component remained. From the above, inhibition of liver fibrosis by elastase may be due to direct affection of elastase to telopetide portion of collagen.

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References

  1. Tsujii, T., Fukuhara, M., Matsumori, T., Tamura, M., Matsui, T. and Morita, T.: Studies on the inhibition of experimental liver fibrosis. 1. Effect of elastase on the liver fibrosis of rats due to carbon tetrachloride. Gastroenterologia Japonica. 9: 145–154, 1974.

    CAS  Google Scholar 

  2. Oda, T. and Yokono, O.: Lysosome in hepatic cell. Metabolism and Disease. 5: 828–834, 1968.

    Google Scholar 

  3. Fishman, W.H.: Application of an improved glucuronidase assay method to the study of human blood β-glucuronidase. J.B.C. 173: 449–456, 1948.

    CAS  Google Scholar 

  4. Anson, M.L.: The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J. Gen. Physiol. 22: 79–89, 1938.

    Article  CAS  Google Scholar 

  5. Frankland, D.M. and Wynn, C.H.: The degradation of acid-soluble collagen by rat-liver preparations. Biochem. J. 85: 276–282, 1962.

    PubMed  CAS  Google Scholar 

  6. Jackson, D.S.: Connective tissue growth stimulated by carrageenin. Biochem. J. 65: 277–284, 1957.

    PubMed  CAS  Google Scholar 

  7. Sakai, T. and Gross, J.: Some properties of the products of reaction of tadopole collagenase with collagen. Biochemistry. 6: 518–528, 1967.

    Article  PubMed  CAS  Google Scholar 

  8. Balo, J. and Banga, I.: The elastolytic activity of pancreatic extracts. Biochem. J. 46: 384–387, 1950.

    PubMed  CAS  Google Scholar 

  9. Grant, H. and Alburn, H.E.: Studies on the collagenase of clostridium histolyticum. Arch. Biochem. Biophys. 82: 245–255, 1959.

    Article  PubMed  CAS  Google Scholar 

  10. Shikata, T., Kanetaka, T. and Sakai, T.: Proliferation of elastic fibers in the liver. Saishin Igaku. 24: 38–45, 1969.

    PubMed  CAS  Google Scholar 

  11. Igarashi, S., Sakai, T., Oda, T. and Fujiwara, K.: Biochemical analysis of the collagen and elastin from cirrhotic human liver. Acta Hepatologica Japo. 13: 691–695, 1972.

    Google Scholar 

  12. Brown, P.C., Consden, R. and Glynn, L.E.: Observation on the shrink temperature of collagen and its variations with age and disease. Ann. Rheum. Disease. 17: 196–208, 1958.

    Article  CAS  Google Scholar 

  13. Schaub, M.C.: A collagen degrading mechanism in the rat uterus during post partum involution. Helv. Physiol. Acta. 21: 227–238, 1963.

    CAS  Google Scholar 

  14. Ungar, H. and Feldman, J.D.: Histologic changes accompanying the absorption of implanted surgical gut in the liver. Am. J. Path. 29: 963–967, 1953.

    PubMed  CAS  Google Scholar 

  15. Fujiwara, K., Sakai, T., Oda, T. and Igarashi, S.: The presence of collagenase in kupffer cells of the rat liver. Biochem. Biophys. Res. Comm. 54: 531–537, 1973.

    Article  PubMed  CAS  Google Scholar 

  16. Nakano, H. and Ito, K.: Hepatic collagenase activity in experimental liver fibrosis. Acta Hepatologica Japo. 13: 161–163, 1972.

    Google Scholar 

  17. Hirayama, C, Hiroshige, K. and Masuya, T.: Hepatic collagenolytic activity in rats after carbontetrachloride poisoning. Biochem. J. 115: 843–847, 1969.

    PubMed  CAS  Google Scholar 

  18. Oshima, Y. and Otaka, Y.: Connective tissue disease. 1st ed., Igakushoin LTD, Tokyo, Japan, 1969, p. 41.

    Google Scholar 

  19. Woessner, J.F. Jr.: Treaties on collagen. Acad. Press, New York, USA, 1968, p. 253.

    Google Scholar 

  20. Lucy, J.A., Dingle, J.T. and Fell, H.B.: Studies on the mode of action of excess of vitamin A. Biochem. J. 79: 500–508, 1961.

    PubMed  CAS  Google Scholar 

  21. Hoffman, P., Linker, A., Lippman, V. and Meyer, K.: The structure of chondroitin sulfate B from studies with flavobacterium enzymes. J. Biol. Chem. 235: 3066–3069, 1960.

    PubMed  CAS  Google Scholar 

  22. Oda, M.: Mechanism of liver fibrosis. Acta Hepatologica Japo. 10: 501–529, 1969.

    Google Scholar 

  23. Hutterer, F.: Degradation of mucopolysaccharides by hepatic lysosomes. Biochim. Biophys. Acta. 115: 312–319, 1966.

    PubMed  CAS  Google Scholar 

  24. Lewis, U.J., Willams, D.E. and Brink, N.G.: Pancreatic elastase purification properties and function. J. Biol. Chem. 222: 705–720, 1956.

    PubMed  CAS  Google Scholar 

  25. Grant, N.H. and Alburn, H.E.: Collagen solubilized by mammalian proteinase. Arch. Biochem. Biophys. 89: 262–270, 1960.

    Article  PubMed  CAS  Google Scholar 

  26. Partridge, S.M. and Davis, H.E.: The chemistry of connective tissue. 3. Component of the soluble proteins derived from elastin. Biochem. J. 61: 21–30, 1955.

    PubMed  CAS  Google Scholar 

  27. Naughton, M.A. and Sanger, F.: Purification and specificity of pancreatic elastase. Biochem. J. 78: 156–163, 1961.

    PubMed  CAS  Google Scholar 

  28. Bornstein, P. and Piez, K.A.: The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the crosslink region of rat skin collagen. Biochemistry. 5: 3460–3473, 1966.

    Article  PubMed  CAS  Google Scholar 

  29. Kang, A.H., Bornstein, P. and Piez, K.A.: The amino acid sequence of peptides from the corsslikage region of rat skin collagen. Biochemistry. 6: 788–795, 1967.

    Article  PubMed  CAS  Google Scholar 

  30. Bornstein, P.: The incomplete hydroxylation of individual prolyl residues in collagen. J. Biol. Chem. 242: 2572–2574, 1967.

    PubMed  CAS  Google Scholar 

  31. Davison, P.F.: Action of proteolytic enzymes on tropocollagen and insoluble collagen. Biochemistry. 5: 301–312, 1966.

    Article  PubMed  Google Scholar 

  32. Sakai, T. Biochemistry of collagen. Saishin Igaku. 24: 156–163, 1969.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The First Department of Internal Medicine, Nara Medical University, 840 Shijocho Kashihara Nara, 634, Japan

    Tadasu Tsujii M.D., Masao Fukuhara, Shinji Fukuda, Tsutomu Matsui, Yoichi Matsuoka, Masahiro Tamura, Tomofumi Morita, Kazuhide Kimura & Wataru Moriya

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  1. Tadasu Tsujii M.D.
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  2. Masao Fukuhara
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  3. Shinji Fukuda
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  4. Tsutomu Matsui
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  5. Yoichi Matsuoka
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  6. Masahiro Tamura
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  7. Tomofumi Morita
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  8. Kazuhide Kimura
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  9. Wataru Moriya
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Tsujii, T., Fukuhara, M., Fukuda, S. et al. Studies on the inhibition of experimental liver fibrosis. Gastroenterol Jpn 10, 215–220 (1975). https://doi.org/10.1007/BF02776655

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  • DOI: https://doi.org/10.1007/BF02776655

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