Summary
The relationship between the electron microscopical structures and the immunoflucrescent appearance of HB antigen found in the hepatocytes of patients with HB antigenemia was carefully studied, with the purpose to clarify the nature of those structures.
The nuclei of hepatocytes, containing numerous virus-like particles, showed positive immunofluorescence by antiserum against the core of Dane particles. The areas in which filamentous structures were observed in the cisternae of endoplasmic reticulums and hyaloplasma, corresponded to orcein or aldehyde-fuchsin positive substances in the light microscope, whereas direct relationship between filamentous structures and HBs antigen was still obscure.
Accordingly, it may be concluded that virus-like particles in the nucleus correspond to core and filamentous structures in the cytoplasma correlate with coat protein of hepatitis B virus.
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References
Brozosko, W.J., Madalinski, K., Krawczynski, K., Nowoslawski, A.: Duality of hepatitis B antigen and its antibody. I. Immunofluorescence studies. J. Infect. Dis., 127: 424–428, 1973.
Barker, L.F., Chisari, F.V., McGrath, P.P.: Transmission of type B viral hepatitis to chimpanzees. J. Inf. Dis. 127: 648–662, 1973.
Shikata, T., Uzawa, T., Yoshiwara, N., Akatsuka, T. and Yamazaki, S.: Staining methods of Australia antigen in paraffin section —Detection of cytoplasmic inclusion bodies-. Japan. J. Exp. Med., 44: 25–36, 1974.
Gerber, M.A., Hadziyannis, S., Vissoulis, C., Schaffner, F., Paronetto, F. and Popper, H.: Electron microscopy and immunoelectron microscopy of cytoplasmic hepatitis B antigen in hepatocytes. Amer. J. Path., 75: 489–502, 1974.
Huang, S.N., Groh, V., Beaudoin, J.G., Dauphinee, W.D., Guttmann, R.D., Morehouse, D.D., Aronoff, A. and Gault, H.: A study of the relationship of virus-like particles and Australia antigen in liver. Human Path., 5: 209–222, 1974.
Shikata, T., Okazaki, K., Kako, M. and Uzawa, T.: Electron microscopical study of cytoplasmic inclusion body in hepatitis B. Japan. J. Exp. Med., 44: 519–530, 1974.
Hadziyannis, S., Gerber, M.A., Vissoulis, C. and Popper, H.: Cytoplasmic hepatitis B antigen in “ground-glass” hepatocytes of carriers. Arch. Path., 96: 327–330, 1973.
Stein, O., Fainaru, M. and Stein, Y.: Virus-like particles in the cytoplasm of the liver of Australia antigen carriers. Amer. J. Dis. Child., 123: 313–314, 1972.
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Shikata, T., Yoshizaki, C., Okazaki, K. et al. HBc and HBs antigen in hepatocytes. Gastroenterol Jpn 10, 199–207 (1975). https://doi.org/10.1007/BF02776653
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DOI: https://doi.org/10.1007/BF02776653