The Indian Journal of Pediatrics

, Volume 51, Issue 6, pp 725–728 | Cite as

Immuno-diagnosis of amoebiasis by antibody detection

  • A. K. Gupta
Symposium: Recent Advances


Serodiagnosis of amoebiasis can be accomplished by indirect hemagglutination, counterimmunoelectrophoresis indirect fluorescent antibody tests or enzyme-linked immunosorbent assay. The specificity and the sensitivity can be improved considerably by applying axenic antigen. Other tests like amoeba immobilization and complement fixation tests are inadequate. Bentonite flocculation and Latex agglutionation tests have similar drawbacks. Agar gel diffusion or immunoelectrophoresis may be used along with the other tests like indirect hemagglutination test, to differentiate the active disease. The detection of coproantibodies may prove useful especially in asymptomatic cases. Antibodies detection in liver abscess aspirates has limited utility, if any, in cases in which serum antibody titres may not be demonstrated at a significant level. Intradermal test (immediate type of hyper-sensitivity) because of its limitations has not much wide application for the diagnosis in routine.

Key words

Entamoeba histolytica serodiagnostic methods intradermal test 


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Copyright information

© Dr. K C Chaudhuri Foundation 1984

Authors and Affiliations

  • A. K. Gupta
    • 1
  1. 1.Department of MicrobiologyPost graduate Institute of Medical Education and ResearchChandigarh

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