Summary
Pancreatic exocrine secretion in the conscious rat is regulated by proteases in the intestine secreted by the pancreas, and cholecystokinin (CCK) is known to be involved in the mechanism. The authors proposed that the release of CCK was regulated by a CCK-releasing factor secreted into the intestinal lumen from the proximal intestine. We isolated and partially purified a CCK-releasing factor from rat small intestine by gel filtration and high performance liquid chromatography. The partially purified CCK-releasing factor increased pancreatic exocrine secretions and plasma CCK concentrations in conscious rats and this activity was abolished after the incubation with trypsin. The bioactivity of the partially purified CCK-releasing factor was confirmed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Green GM, Lyman RL: Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor induced hypersecretion in rats. Proc Soc Exp Biol Med 1972;140:6–12.
Folsch UR, Cantor P, Wilms HM, et al: Role of cholecystokinin in the negative feedback control of pancreatic enzyme secretion in conscious rats. Gastroenterology 1987;87:542–549.
Louie DS, May D, Miller P, et al: Cholecystokinin mediates feedback regulation of pancreatic enzyme secretion in rats. Am J Physiol 1986;250.G252–259.
Nakamura R, Miyasaka K, Funakoshi A, et al: Interactions between bile and pancreatic juice diversions on cholecystokinin release and pancreas in conscious rats. Proc Soc Exp Biol Med 1989;192:182–186.
Lu L, Louie DS, Owyang C: Extraction and characterization of CCK-releasing peptide mediating feedback regulation of pancreatic secretion. Am J Physiol 1989;256:G430-G435.
Miyasaka K, Guan D, Liddle RA, et al: Feedback regulation by trypsin: Evidence of intraluminal CCK-releasing peptide Am J Physiol 1989;257:G175-G181.
Miyasaka K, Nakamura R, Funakoshi A, et al: Stimulatory effect of monitor peptide and human pancreatic secretory trypsin inhibitor on pancreatic secretion and cholecystokinin release in conscious rats. Pancreas 1989;4:139–144.
Erlanger BF, Kokowsky N, Cohen W: The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem 1961;95:271–278.
Lowry OH, Rosebrough NJ, Farr AL, et al: Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–275.
Liddle RA, Goldfine ID, Williams JA: Bioassay of plasma cholecystokinin in rats: Effects of food, trypsin inhibitor, and alcohol. Gastroenterology 1984;87:542–549.
Iwai K, Fushiki T, Fukuoka S: Pancreatic enzyme secretion mediated by novel peptide: Monitor peptide hypothesis. Pancreas 1989;3:720–728.
Miyasaka K, Funakoshi A: Comparative effects between cholecystokinin-releasing peptide and monitor peptide on cholecystokinin release and pancreatic exocrine secretion in conscious rats. Jpn J Gastroenterol 1991;88:1147.
Guan DH, Ohta T, Tawil T, et al: CCK-releasing activity of rat intestinal secretion: effect of atropine and comparison with monitor peptide. Pancreas 1990;5:677–684.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miyasaka, K., Funakoshi, A. Isolation and bioactivity of putative cholecystokinin-releasing peptide from rat small intestinal mucosa. Gastroenterol Jpn 27, 83–87 (1992). https://doi.org/10.1007/BF02775068
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02775068