Summary
Pancreatic elastase-induced degradation of some plasma proteins was studied in an in vitro model. The digestion was correlated with the degree of saturation of the α l-proteinase inhibitor (α1PI) and also with varying amounts of secretory leucocyte proteinase inhibitor (SLPI). SLPI was found to inhibit pancreatic elastase showing a Ki of about 10-7 M for the complex. On the addition of human pancreatic elastase to plasma cleavage of C3, kininogen, fibrinogen and fibronectin was observed when the α1 PI approached saturation. In the present in vitro model it was possible to block the cleavage of the four plasma proteins, mentioned above completely with SLPI. Addition of the inhibitor also decreased the consumption of α1PI.
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Geokas MC, Rinderknecht H, Swanson V, et al. The role of elastase in acute hemorrhagic pancreatitis in man. Laboratory investigation 1968;219:235–239.
Gustavsson EL, Ohlsson K, Olsson AS. Interaction between human pancreatic elastase and plasma protease inhibitors. HoppeSeyler’s Z Physiol Chem 1980;361:169–176.
Håkansson HO, Ohlsson K. Interactions in vitro and in vivo between human and porcine cationic pancreatic elastase and plasma protease inhibitors. Biol Chem Hoppe-Seyler 1988;369:309–315.
Laine A, Davril M, Rabaud M, et al. Human serum alpha 1-antichymotrypsin is an inhibitor of pancreatic elastase. Eur J Biochem 1985;151:327–331.
Fritz H, Wunderer G. Biochemistry and applications of aprotinin, the kallikrein inhibitor of bovine organs. Artzneimittel Forsch 1983;33:479–494.
Tamura Y, Hirado M, Okamura K, et al. Synthetic inhibitors of trypsin, plasmin, kallikrein, thrombin, Clr and Cl esterase. Biochem Biophys Acta 1977;484:417–422.
Thompson RC, Ohlsson K. Isolation, properties, and complete amino acid sequence of human secretory leucocyte protease inhibitor, a potent inhibitor of leucocyte elastase. Proc Natl Acad Sci USA1986;83:6692–6696.
Heinzel R, Appelhans H, Gassen HG. The Neutrophil Elastase-Cathepsin G inhibitor of human mucus tissues and secretions (Antileukoprotease, HUSI-1) In: Taylor JC, Mittman CH, eds. Complete Primary structure as revealed by protein and DNA sequencing. New York: Academic Press, 1986;297–306.
Ohlsson K, Rosengren M, Steuer G. Structure, genomic organization, and tissue distribution of human secretory leucocyteprotease inhibitor (SLPI) In: Taylor JC, Mittman CH, eds. a potent inhibitor of neutrophil elastase. New York: Academic Press, 1986;307–324.
Schiessler H, Arnhold M, Ohlsson K, et al. Inhibitors of acrosin and granulocyte proteinases from human genital tract secretions. Hoppe-Seyler’s Z Physiol Chem 1976;357:1251–1260.
Ohlsson K, Linder C, Rosengren M. Monoclonal antibodies specific for neutrophil proteinase 4. Hoppe-Seyler’s Z Physiol Chem 1990;371:549–555.
Ohlsson K, Olsson AS. Purification and partial characterization of human pancreatic elastase. Hoppe-Seyler’s Z Physiol Chem 1976;357:1153–1161.
Lassen M. Heat denaturation of plasminogen in the fibrin plate method. Acta Physiologica Scandinavica 1952;27:371–376.
Bieth J. In vivo significance of kinetic constants of macromolecular proteinase inhibitors. Biochem Med 1984;32:387–397.
Brandslund I, Teisner B, Hyltoft-Petersen P, et al. Development and clinical application of electroimmunoassays for the direct quantification of complement C3 split products C3c and C3d. Scand J Clin Lab Invest 1984;44 (Suppl 168):57–73.
Ganrot PO. Crossed immunoelectrophoresis. Scand J Clin Lab Invest 1972;29(Suppl 124):39–41.
Balldin A, Gustafsson EL, Ohlsson K. Influence of plasma protease inhibitors and trasylol on trypsin-induced bradykinin-release in vitro and in vivo. Eur Surg Res 1980; 12:260–269.
Balldin A, Eddeland A, Ohlsson K. Studies on the role of the plasma protease inhibitors on in vitro C3-activation and in acute pancreatitis. Scand J Gastroenterol 1981;16:603–609.
Håkansson HO. Studies on the possible role of pancreatic cationic elastase in acute pancreatitis. Thesis, University of Lund, Sweden 1991.
Satake K, Chung YS, Yoshimoto T, et al. Radioimmunoreactive serum elastase levels and histologic changes during experimental pancreatitis in rats. Arch Surg Res 1982;117:777–780.
Håkansson HO, Borgström A, Ohlsson K. Pancreatic cationic elastase in porcine experimental pancreatitis. Eur Surg Res 1991;23:73–84.
Ohlsson K, Eddeland A. Release of proteolytic enzymes in bileinduced pancreatitis in dogs. Gastroenterology 1975;369:668–675.
Schoenemann J. Elastase des Pankreas und ihre Inhibitoren. Georg Stuttgart: Thieme Verlag 1978;14:1–50.
Hjelmqvist B, Ohlsson K, Aronsen KF. Protease-antiprotease imbalance, hemodynamic and regional blood flow changes in experimental pancreatitis. Scand Gastroenterol 1986;21:8–11.
Ohlsson K, Olsson R, Björk P. Local administration of human pancreatic secretory trypsin inhibitor prevents the development of experimental acute pancreatitis in rats and dogs. Scand J Gastroenterol 1989;24:693–704.
Ohlsson K, Balldin G, Bohe M, et al. Pancreatic proteases and antiproteases in pancreatic disease; biochemical, pathophysiological and clinical aspects. International Journal of Pancreatology 1988;3:67–78.
Lasson Å, Balldin G, Ohlsson K. Leucocyte elastase al-proteinase inhibitor complexes may diagnose pancreatic abscesses early. Scand J Gastroenterol 1986;21:221–224.
Bergenfeldt M, Björk P, Ohlsson K, et al. Release of immunoreactive canine leucocyte elastase, normally and in endotoxin and pancreatic shock. Scand J Clin Invest 1990;50:35–42.
Björk P, Axelsson L, Bergenfeldt M, et al. Influence of plasma protease inhibitors and the secretory leucocyte protease inhibitor on. Scand J Clin Invest 1988;48:205–211.
Laurent P, Rabaud M, Bieth JB. Inhibition of free and elastinbound human pancreatic elastase by human bronchial inhibitor. Biochemical pharmacology 1987;536:765–767.
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Håkansson, HO., Ohlsson, K. Influence of plasma proteinase inhibitors and the secretory leucocyte proteinase inhibitor on pancreatic elastase-induced degradation of some plasma proteins. Gastroenterol Jpn 27, 652–656 (1992). https://doi.org/10.1007/BF02774981
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DOI: https://doi.org/10.1007/BF02774981