Summary
The authors previously reported the ring-like distribution of IgG-containing cells around the lymph follicle in both the small and large intestine. In the present study, the distribution of IgG subclasscontaining cells around the lymph follicle was examined in both the small and large intestine, including Peyer’s patches, by the indirect immunoperoxidase staining method. A ring-like distribution of IgG subclass-containing cells was observed in 14 out of 21 lymph follicles including 4 Peyer’s patches (66.7%) in the terminal ileum, and 4 out of 7 (57.1%) in the large intestine. The percentages of IgG1-, IgG2-, IgG3-, and IgG4- containing cells around the lymph follicle in the terminal ileum were 27.6±11.6%, 51.9±15.4%, 16.5±10.2%, and 4.0±3.1%, respectively, and those in the large intestine were 22.9±15.4%, 47.4±14.9%, 11.2±4.1%, and 18.5±7.7%, respectively. Thus, among the IgG subclasses, IgG2-containing cells showed the most frequent ring-like distribution around the lymph follicle. The IgG2 predominance around the lymph follicle in the intestine was markedly different from reported IgGl predominance in the lamina propria in the large intestine. This difference in IgG subclass distribution may suggest different origins of the IgG subclass-containing cells in the lamina propria and in areas around the lymph follicle.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sminia T, Plesch BEC. An immunohistochemical study of cells with surface and cytoplasmic immunoglobulins in situ in Peyer’s patches and lamina propria of rat small intestine. Virchows Arc 1982;40:181–189.
Bjerke K, Brandtzaeg P, Rognum TO. Distribution of immunoglobulin producing cells is different in normal human appendix and colon mucosa. Gut 1986;27:667–674.
Chiba M, Ohta H, Iizuka M, et al. Ring-like distribution of IgGcontaining cells around the lymph follicle in the human intestine. Gastroenterol Jpn 1987;22:703–708.
Bjerke K, Brandtzaeg P. Terminal differentiated human intestinal B cells IgA and IgG subclass-producing immunocytes in the distal ileum, including Peyer’s patches, compared with lymph nodes and palatine tonsils: Scand J Immunol 1990;32:61–67.
Iizuka M. IgG subclass-containing cells in the human large bowel of normal controls, non-IBD colitis, and ulcerative colitis. Gastroenterol Jpn 1990;25:24–31.
Konno K, Huruyama S, Hirane T, et al. Immunohistochemical study of IgG, IgA subclass-producing cells in the colonic mucosa in inflammatory bowel disease. Digestive Organ and Immunology 1989;22:82–86. (in Japanese)
Chiba M, Ohta H, Nagasaki A, et al. Lymphoid cell subsets in normal human small intestine. Gastroenterol Jpn 1986;21:336–343.
Jefferis R, Reimer CB, Skvaril F, et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses: Results of an IUIS/WHO collaborative study. Immunol Lett 1985;10:223–252.
Lowe J, Bird P, Hardie D, et al. Monoclonal antibodies(Mc Abs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity. Immunology 1982;47:329–336.
Bjerke K, Brandtzaeg P. Immunoglobulinand J chain-producing cells associated with lymphoid follicles in the human appendix, colon and ileum, including Peyer’s patches. Clin Exp Immunol 1986;64:432–441.
Schur PH. IgG subclasses-a review. Ann Allergy 1987;58:89–96.
Mayumi M, Kuritani T, Kubagawa H, et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J Immunol 1983;130:671–677.
Conley ME, Brown P, Bartelt MS. IgG subclass potential of surface IgM-negative and surface IgM-positive human peripheral blood B cells. Clin Immunol and Immunopathol 1987;43:211–222.
Pappo J, Owen RL. Absence of secretary component expressing by epithelial cells overlying rabbit gut-associated lymphoid tissue. Gastroenterology 1988;95:1173–1177.
Kett K, Rognum TO, Brandtzaeg P. Mucosal subclass distribution of immunoglobulin G-producing cells is different in ulcerative colitis and Crohn’s disease of the colon. Gastroenterology 1987;93:919–924.
Snapper CM, Finkelman FD, Paul WE. Regulation of IgGl and IgE production by Interleukin 4. Immunol Rev 1988;102:51–75.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iizuka, M., Chiba, M., Ishii, N. et al. IgG subclass-containing cells around the lymph follicle in the human intestine. Gastroenterol Jpn 27, 611–616 (1992). https://doi.org/10.1007/BF02774975
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02774975