Summary
Adenosine triphosphatase (ATPase) activity and acid phosphatase activity in lymphocytes from patients with carcinoma of the gastrointestinal tract were determined in order to investigate whether or not changes in these enzyme activities has any relation to the immune reactivity of carcinoma-bearing patients. In patients with a performance status of more than 60%, the mean value of the total ATPase activity in lymphocytes differed little from that in controls, but the mean value of the oligomycin-sensitive ATPase activity decreased as compared to that in the controls. On the other hand, the mean values of the activities of both free and total acid phosphatase in lymphocytes increased as compared to those in controls. The mean values of the activities of both ATPase and acid phosphatase in lymphocytes from patients whose performance status was less than 50% decreased as compared to those from controls and patients whose performance status was more than 60%. The change of the activities of both ATPase and acid phosphatase in lymphocytes has relation to that of the immunological parameters of the patients with carcinoma of the gastrointestinal tract. These results indicate that both ATPase and acid phosphatase in lymphocytes may play an important role in the immune mechanism.
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Bessey OA, Lowry OH, Brock MJ: A method for the rapid determination of alkaline phosphatase with five cubic milimeters of serum. J Biol Chem 163: 321, 1946
de Duve C, Pressmans BC, Gianetto R, Wattiaux R, Appelmans F: Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J 60: 604, 1955
Fiske CH, Subbarow Y: The colorimetric determination of phosphorous. J Biol Chem 66: 375, 1925
Huijing F, Slater EC: The use of oligomycin as an inhibitor of oxidative phosphorylation. J Biochem 49: 493, 1961
Hersh EM, Murphy SG, Quesada JR, Gutterman JU, Gaschwind CR, Morgan J: Effect of immunotherapy with Corynebacterium parvum and methanol extraction residue of BCG administered intravenously and methanol extraction residue of BCG administrated intravenously on host defense function in cancer patients. J Natl Cancer Inst 66: 993, 1981
Karnofsky DA: Clinical evaluation of anticancer drugs. GANN Monogr 2: 223, 1968
Kiyohashi A, Taniguchi T, Kitsuta T, Toyama K, Okabe H: Changes of adenosinetriphosphatase activities in lymphocytes from patients with carcinomas of the gastrointestinal tract. Gastroent Jpn 16: 275, 1981
Skou J: Preparation from mammalian brain and kidney of the enzyme system involved in active transport ofNa+ and K+. Biochim Biophys Acta 58: 318, 1962
Sone S, Yata K, Tsubura E: Rebound phenomenon of phytohemogglutinin skin reactivity in leukemia and malignant lymphoma after chemotherapy. GANN 68: 483, 1977
Saijo N, Niitani H, Taniguchi T, Kawase I, Kimura K: Changes of enzyme activities in spleen lymphocytes from tumor-bearing rats. GANN 66: 253, 1975
Saijo N, Niitani H, Chikama M, Taniguchi T, Kimura K: Effect of propionibacterium acnes or BCG on enzyme activities in spleen lymphocytes from tumor-bearing rats. GANN 69: 345, 1978
Taniguchi T, Saijo N, Kawase I, Niitani H: Enzyme activities in spleen lymphocytes of tumor-bearing rats, and effect of Propionibacterium acnes, BCG, and Toxohormone on the enzyme activities in spleen lymphocytes of rats. Cell Mol Biol 27: 7, 1981
Taniguchi T, Kiyohashi A, Kitsuta T, Okabe H: Changes of ATPase activities in lymphocytes from patients with carcinomas of the gastrointestinal tract. GANN 72: 333, 1981
Taniguchi T, Kitsuta T, Kiyohashi A, Okabe H: Changes of enzyme activities in lymphocytes from patients with carcinomas of the gastrointestinal tract. In Proceedings of 13th International Cancer Congress, Seattle, p 199, 1982
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Kitsuta, T., Taniguchi, T., Kiyohashi, A. et al. Changes of enzyme activities recognized in lymphocytes from patients with carcinoma of the gastrointestinal tract. Gastroenterol Jpn 18, 365–371 (1983). https://doi.org/10.1007/BF02774954
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DOI: https://doi.org/10.1007/BF02774954