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Hepatocyte plasma membrane antigens. I. Determination of antibodies bound to the hepatocyte plasma membrane by125I-protein a binding assay

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Summary

Anti-liver-specific membrane lipoprotein (anti-LP-1) and anti-Tamm-Horsfall glycoprotein (anti-THGP) rabbit antibodies were found to bind to Chang liver cells, a cultured human hepatocyte cell line, and PLC/PRF/5, a hepatoma cell line. The antibodies bound were determined by an immunofluorescence staining and a semiquantitative125I-protein A binding assay. The125I-protein A binding assay was successfully adapted to determine anti-hepatocyte plasma membrane antibodies in sera of patients with lupoid hepatitis and chronic active hepatitis. The percentage of125I-protein A bound in 10 normal subjects were 1.5 ± 0.4 (mean ± standard deviation) for PLC/PRF/5 and 1.6 ± 0.6 for Chang liver cell, while those in 2 patients with lupoid hepatitis were 7.2±0.3, 5.9±0.1, and those in 8 patients with chronic active hepatitis 3.9±1.3, 3.2±1.5, respectively. Furthermore, a blocking study revealed that LP-1 and THGP were partially involved in antigen sites recognized with anti-hepatocyte plasma membrane antibodies in sera of a patient with lupoid hepatitis. The retaining ability of antibody binding to the hepatocytes after the absorption with non-hepatocyte cells suggested the presence of antibodies specific for the hepatocyte plasma membrane in the patient’s serum.

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  1. First Department of Internal Medicine, Okayama University Medical School, 5-1, Shikata-cho 2-chome, 700, Okayama, Japan

    Kohsaku Sakaguchi M.D.

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  1. Kohsaku Sakaguchi M.D.
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Sakaguchi, K. Hepatocyte plasma membrane antigens. I. Determination of antibodies bound to the hepatocyte plasma membrane by125I-protein a binding assay. Gastroenterol Jpn 18, 330–338 (1983). https://doi.org/10.1007/BF02774950

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  • DOI: https://doi.org/10.1007/BF02774950

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