Summary
Leukocytes from patients with gastric, colorectal or benign gastrointestinal disease, as well as those from normal control subjects, were tested by the leukocyte migration inhibition test (LMIT), using five different allogeneic 3 M KC1 extracts of gastric cancer extracts. In LMIT with a single tumor extract, a pathological MI was found in 48% of 79 gastric cancer patients, a significantly higher figure than in the three other groups of patients (4–21 %). In the panel mode of LMIT, that is, testing each blood sample with five different tumor extracts, 62/79 (79%) of patients with gastric cancer showed a positive reaction. Positive reactivity was significantly higher in patients with gastric cancer than in patients in the other groups and the frequency was higher F in stage IV cases than in stage I-III cases, although the differences were not significant. In addition, the frequency of the positive reaction was unrelated to the degree of differentiation in adenocarcinoma. Positive reactivity increased in inverse proportion to the degree of nuclear grade (NG) (84% in NG I, 80% in NG II and 64% in NG III), but the correlation was not statistically significant.
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Anderson V, et al: Effect of autologous mammary tumor extracts on human leukocyte migration in vitro. Int J Cancer 5: 357, 1970
McCoy JL, et al: Inhibition of leukocyte migration by tumor-associated antigens in soluble extracts of human malignant melanoma. J Natl Cancer Inst 55: 19, 1975
Zöller M, et al: Leukocyte migration studies in gastric cancer detection: An approach toward improved specificity and sensitivity. J Natl Cancer Inst 58: 897, 1977
Zöller M, et al: Colorectal cancer diagnosis by a direct leukocyte migration test using a panel of tumor extracts. Cancer Immunol Immunother 2: 257, 1977
Kojima O, et al: Studies on cell-mediated immunity in patients with colorectal cancer: Leukocyte migration inhibition test using allogeneic 3 M KG extract. Aust NZ J Surg 51:434, 1981
Reisfeld RA, et al: Salt extraction of soluble HL-A antigens. Science 172: 1134, 1971
Lowry OH, et al: Protein measurement with the folin phenol reagent. Biol Chem 193: 265, 1951
Clausen JE: Tuberculin-induced migration inhibition of human peripheral leukocytes in agarose medium. J Acta Allerg(Kbh)26: 56, 1971
Weisbart RH, et al: Migration enhancement factor: A new lymphokine. Proc Nati Acad Sci (Wash) 71: 875, 1979
Jpanese Research Society for Gastric Cancer: The general rules for the gastric cancer study in surgery and pathology. Jpn J Surg 3: 61, 1973
Black MM, et al: Survival in breast cancer cases in relation to structure of primary tumor and regional lymph nodes. SGO 100: 543, 1955
Black MM, et al: Cellular hypersensitivity to breast cancer. Assessment by a leukocyte migration procedure. Cancer 33: 952, 1974
Lurie BB, et al: Diagnosis and prognosis in colon based on a profile of immune reactivity. J Natl Cancer Inst 54: 319, 1975
McCoy JL: Clinical application of assays of leukocyte migration inhibition, Herberman RB, Mclntire KR (ed), Immunodiagnosis of cancer, New York, Dekker, 1979, pp 979–998
Cochran AJ: In vitro testing of the immune response. Castro JE (ed), Immunological aspects of cancer, Lan caster (England), MTP press Ltd, pp 219–266, 1978
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Kojima, O., Uehara, Y., Majima, T. et al. Immunoassessment of patients with gastric cancer. Using leukocyte migration inhibition (LMI) test. Gastroenterol Jpn 18, 303–309 (1983). https://doi.org/10.1007/BF02774946
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DOI: https://doi.org/10.1007/BF02774946