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Immunological studies on crohn’s disease V. Enumeration of circulating lymphocytes subsets using monoclonal antibodies

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Summary

We found normal levels of suppressor cell activity and reduced natural killer (NK) and antibody dependent cellmediated cytotoxicity (ADCC) activities in patients with Crohn’s disease (CD). To further characterize these activities, studies were carried out using monoclonal antibodies. There were no changes in the proportion of OKT4 + (helper/inducer T cells), OKT8 + or Leu 2 + (suppressor/cytotoxic T cells) and Leu 7 + cells (large granular lymphocytes: LGL, NK + K cells), thereby suggesting that suppressor cell activity in CD is likely to be normal both in function and in number, and that depressed NK and ADCC activities are not due to a reduction in the number of NK or K cells but rather to functional defects. Using a double staining method, we noted a low percentage of both Leu 2 and Leu 7 positive cells in CD.

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Authors and Affiliations

  1. The First Department of Internal Medicine, School of Medicine, Fukuoka University, Nanakuma 7-45-1, Jonan-ku, 814-01, Fukuoka, Japan

    Nobuo Okabe M.D., Koichi Fujita M.D., Misao Yamasaki M.D. & Tsuneyoshi Yao M.D.

  2. Department of Bacteriology, National Defence Medical College, 359, Saitama, Japan

    Sumiaki Tsuru M.D.

Authors
  1. Nobuo Okabe M.D.
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  2. Koichi Fujita M.D.
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  3. Misao Yamasaki M.D.
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  4. Tsuneyoshi Yao M.D.
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  5. Sumiaki Tsuru M.D.
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Okabe, N., Fujita, K., Yamasaki, M. et al. Immunological studies on crohn’s disease V. Enumeration of circulating lymphocytes subsets using monoclonal antibodies. Gastroenterol Jpn 20, 431–435 (1985). https://doi.org/10.1007/BF02774779

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  • DOI: https://doi.org/10.1007/BF02774779

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