Antibody to liver-specific lipoprotein in acute and chronic liver diseases. its quantitative assay with monoclonal antibody, but without the use of liver-specific lipoprotein

Summary

A double-antibody radioimmunoassay was developed to detect antibody to human liver-specific membrane lipoprotein (anti-LSP antibody) in patients’ serum. Anti-human LSP monoclonal antibody was labeled with125I and anti-anti-LSP antiserum raised in rabbits was used as the first antibody in the assay. Anti-LSP antibody level was quantitatively measured and the assay was shown to be specific. Anti-LSP antibody was found in 5/8 patients with type B acute viral hepatitis (AVH), 3/7 patients with type A AVH, 1/6 patients with non-A, non-B AVH, 10/17 patients with chronic active hepatitis (CAH), 6/16 patients with chronic persistent hepatitis, 13/16 patients with active cirrhosis of the liver and 7/19 patients with primary nonhepatic autoimmune diseases such as glomerulonephritis, systemic lupus erythematosus and rheumatoid arthritis. The mean levels of anti-LSP were increased in patients with cirrhosis of the liver (p<0.01), CAH (p<0.05) and AVH (p<0.05) when compared with that of normal individuals. However, the frequencies of anti-LSP did not depend on HBsAg status. The data showed that anti-LSP antibody can be detected without the use of LSP preparation although it is also found in patients with primary nonhepatic autoimmune diseases.

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Correspondence to Shinichi Kakumu M.D..

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Kakumu, S., Yoshioka, K. & Tsubouchi, A. Antibody to liver-specific lipoprotein in acute and chronic liver diseases. its quantitative assay with monoclonal antibody, but without the use of liver-specific lipoprotein. Gastroenterol Jpn 20, 216–221 (1985). https://doi.org/10.1007/BF02774707

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Key words

  • Serum antibody to liver-specific lipoprotein
  • Liver disease
  • RIA with monoclonal antibody