Summary
Duodenal juice collected after administration of Boot’s pancreozymin and secretin to patients with various pancreatic diseases was subjected to deoxyribonuclease I (DNase I) assay, as well as measurements of total volume, amylase output and maximum bicarbonate concentration. It was observed that the DNase I output is well correlated with each of the three latter factors. The DNase I output was much lower in patients with chronic pancreatitis or pancreatic cancer than in control subjects, and DNase I output was even found to be low in patients suspected of having chronic pancreatitis, who did not give abnormal results with other assay methods. These results imply that DNase I output may be a good indicator of exocrine function of the pancreas, and thus may be useful for early detection of pancreatic diseases.
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References
Sun DCH, et al: Pancreozymin-Secretin test. Gastroenterology 38: 570–581, 1960
Sun DCH: Normal values for Pancreozymin-Secretin test. Gastroenterology 44: 602–606, 1963
Laskowski M Sr: Deoxyribonuclease I. in “The Enzymes”. Vol 4, edited by Boyer PD, Lardy H, Myrback K, Academic Press, New York, 1971, p 289–311
Bernardi G: Spleen acid deoxyribonuciease. in “The Enzymes”. Vol 4, edited by Boyer PD, Lardy H, Myrback Academic Press, New York, 1971, p 271–287
Lindahl T, et al: Properties of deoxyribonuclease III from mammalian tissues. J Biol Chem 244: 5014–5019, 1969
Lindahl T, et al: Deoxyribonuclease IV: A new exonuclease from mammalian tissues. Proc Nati Acad Sei U.S.A. 62: 597–603, 1969
Allfrey, V, et al: Some aspects of the deoxyribonuclease activities of animal tissues. J Gen Physiol 36: 227–241, 1952
Funakoshi A, et al: Purification and properties of human pancreatic deoxyribonuclease I. J Biochem 82: 1771–1777, 1977
Abe M: The exocrine pancreatic function test. Jpn J Gastroenterology 68: 630–631, 1971
Natelson S: Routine use of ultramicro methods in the clinical laboratory. Am J Clin Pathol 21: 1153–1172, 1959
Somogyi M: Micromethods for the estimation of diastase. J Biol Chem 125: 399–414, 1969
Anai M, et al: A deoxyribonuclease which requires nucleoside triphosphate fromMicrococcus lysodeikticus. J Biol Chem 245: 767–774, 1970
Dreiling DA, et al: The measurement of pancreatic secretory function, in “Ciba Foundation Symposium on the Exocrine Pancreas”, edited by de Reuck, A.V.S., and Cameron, M.P., J. & A. Churchill Ltd London 1962, p 225–252
Hotz J, et al: Reserve capacity of the exocrine pancreas. Digestion 9: 212–223, 1973
DiMagno EP, et al: Relations between pancreatic enzyme output and malabsorption in severe pancreatic insufficiency. New Eng J Med 288: 813–8151973
Kowlessar OD, et al: Desoxyribonuclease I activity in pancreatic disease. J Clin Invest 35: 1325–1330, 1958
Wroblewski F, et al: Presence of desoxyribonuclease activity in human serum. Proc Soc Exp Biol 74: 443–445, 1950
Kurnick NB: Desoxyribonuclease activity of sera of man and some other species. Arch Biochem Biophys 43: 97–107, 1953
Lazarides E, et al: Actin is the naturally occurring inhibitor of deoxyribonuclease I. Proc Nati Acad Sei U.S.A. 71: 4742–4746, 1974
Reddi KK, et al: Elevated serum ribonuclease in patients with pancreatic cancer. Proc Nati Acad Sei U.S.A. 73: 2308–2310 1976
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Funakoshi, A., Tsubota, Y., Wakasugi, H. et al. Clinical studies on human pancreaticdeoxyribonuclease I. Gastroenterol Jpn 14, 48–54 (1979). https://doi.org/10.1007/BF02774604
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DOI: https://doi.org/10.1007/BF02774604