Summary
When peripheral lymphocytes from patients with drug-induced allergic intrahepatic cholestasis were stimulated with a specific drug in vitro in the presence of a liver microsome fraction or soluble liver specific antigen fraction, lymphokine production was seen in many cases. By the injection of culture supernatant of stimulated lymphocytes into the mesentery vein of dogs, cholestasis was induced in the liver, chiefly in the central zones of lobules. However, no cholestasis could be observed in dogs administered the supernatants of lymphocyte cultures prepared from normal individuals in the presence of drugs. Moreover, only slight swelling of the hepatocytes was observed in the liver when normal lymphocytes were stimulated with PHA-P and culture supernatant was injected into the mesentery vein of dogs. These results suggest that sensitized lymphocytes may produce a factor (or factors) by stimulation with a specific drug-carrier and this factor (or factors) causes cholestasis in the liver.
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References
Sarkany I: Lymphocyte transformation in drug hypersensitivity. Lancet 1: 743–745, 1967
Rocklin RE, David JR: Detection in vitro of cellular hypersensitivity to drugs. J Allergy 48: 276–282, 1971
Paronetto F, Popper H: Lymphocyte stimulation induced by halothane in patients with hepatitis following exposure to halothane. New Eng J Med 283: 277–280, 1970
Mizoguchi Y, et al: Detection of hypersensitivity to drugs by lymphocyte culture in drug-induced allergic hepatitis. Gastroenterologia Japonica 10: 300–306, 1975
Mizoguchi Y, et al: Immunological studies on druginduced allergic hepatitis. Gastroenterologia Japonica 11: 105–110, 1976
Meyer zum Buschenfelde: Untersuchungen uber die immunbiologische Bedeutung loslicher Leberproteine. Z Ges Exp Med 148: 131–163, 1968
Park BH, Good RA: A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus dependent cells. Proc Nati Acad Sei 69: 371–373, 1972
Paty DW, Hughes D: Lymphocyte transformation using whole blood culture. An analysis of responses. J Immunol Methods 2: 99–114, 1972
Dumonde DC, Maini RN: The clinical significance of mediator of cellular immunity. Clin Allergy 1: 123–139, 1971
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Mizoguchi, Y., Monna, T., Yamamoto, S. et al. Studies on drug-induced allergic intrahepatic cholestasis. Gastroenterol Jpn 14, 19–23 (1979). https://doi.org/10.1007/BF02774600
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DOI: https://doi.org/10.1007/BF02774600